Characterization and application of lytic bacteriophages against campylobacter jejuni isolated from poultry in Japan

Munenori Furuta, Takayuki Nasu, Kouichi Umeki, Duc Hoang Minh, Ken-Ichi Honjoh, Takahisa Miyamoto

研究成果: ジャーナルへの寄稿記事

1 引用 (Scopus)

抄録

The aim was to isolate Campylobacter jejuni- specific lytic phages from meats on the market in Japan. These phages were effectively isolated from 13 of 15 (86.7%) retail chicken meat samples (skin and liver) by the enrichment method using Preston Campylobacter Selective Enrichment Broth and 10 host Campylobacter strains. Among the 26 phage isolates, 14 were extracted by means of C. jejuni L26 as a host strain. Phage PHC10 showed the broadest lytic spectrum: Active against 67.4% of the 46 C. jejuni strains tested. The other phage isolates showed different lytic spectra. Because phages PHC5, PHC10, PHC19, PHC22, and PHC25 possess an icosahedral head and a contracted tail, they seem to be members of the Myoviridae family. Effects of 19 phage isolates on viability of C. jejuni were investigated. These phages reduced viable counts of C. jejuni by 1-3 log after 6-12 h of incubation at 42C as compared to the initial counts. The C. jejuni L26 was found to be suitable as a host because of the wide hosting range. The phages isolated in this study seem to be promising biocontrol agents against C. jejuni in food.

元の言語英語
ページ(範囲)213-221
ページ数9
ジャーナルBiocontrol Science
22
発行部数4
DOI
出版物ステータス出版済み - 1 1 2017

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Campylobacter jejuni
Poultry
Bacteriophages
Japan
Campylobacter
Meat
Myoviridae
Tail
Chickens
Food
Skin
Liver

All Science Journal Classification (ASJC) codes

  • Applied Microbiology and Biotechnology
  • Public Health, Environmental and Occupational Health

これを引用

Characterization and application of lytic bacteriophages against campylobacter jejuni isolated from poultry in Japan. / Furuta, Munenori; Nasu, Takayuki; Umeki, Kouichi; Minh, Duc Hoang; Honjoh, Ken-Ichi; Miyamoto, Takahisa.

:: Biocontrol Science, 巻 22, 番号 4, 01.01.2017, p. 213-221.

研究成果: ジャーナルへの寄稿記事

Furuta, Munenori ; Nasu, Takayuki ; Umeki, Kouichi ; Minh, Duc Hoang ; Honjoh, Ken-Ichi ; Miyamoto, Takahisa. / Characterization and application of lytic bacteriophages against campylobacter jejuni isolated from poultry in Japan. :: Biocontrol Science. 2017 ; 巻 22, 番号 4. pp. 213-221.
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