Characterization and distribution of bone marrow-derived cells in mouse cornea

Takahiro Nakamura, Fumihiko Ishikawa, Koh Hei Sonoda, Toshio Hisatomi, Hong Qiao, Jun Yamada, Mitsuhiro Fukata, Tatsuro Ishibashi, Mine Harada, Shigeru Kinoshita

研究成果: ジャーナルへの寄稿記事

91 引用 (Scopus)

抄録

PURPOSE. Bone marrow (BM)- derived stem cells are thought to possess extensive differentiation capacity. The present study was conducted to investigate the characteristics and distribution of these cells in the normal mouse cornea. METHODS. BM cells and BM-derived hematopoietic stem/progenitor cells (HSCs) from enhanced GFP (eGFP) transgenic mice (lin-, Sca-1+) were intravenously transplanted into irradiated wild-type C57BL/6 mice. At 4 to 6 months after transplantation, the mice were killed, and their whole corneas examined by histologic and immunohistochemical methods (CD11c, CD11b, and CD45). RESULTS. At 2 weeks after BM cell transplantation, GFP+ cells gradually migrated into the cornea from the limbal area. At 2 to 6 months, they were distributed over the entire cornea. In cross sections of whole cornea, GFP+ cells comprised 27.3% ± 11.1% (BM) and 24.0% ± 8.01% (HSC) of total cells in the peripheral corneal stroma. In the center of the corneal stroma, GFP+ cells were 7.58% ± 2.63% (BM) and 8.06% ± 1.76% (HSC) of total cells. Immunohistochemistry showed that GFP+ CD11c+, CD11b +, CD11c-, and CD11b- cells occupied the entire corneal stroma. CONCLUSIONS. The present study provides direct evidence of the distribution of BM-derived cells in the mouse cornea. Immunohistochemical study showed that some of these cells are BM-derived antigen-presenting cells such as dendritic cells and macrophages. Some elements of BM-derived cells may continue to exist in the corneal stroma.

元の言語英語
ページ(範囲)497-503
ページ数7
ジャーナルInvestigative Ophthalmology and Visual Science
46
発行部数2
DOI
出版物ステータス出版済み - 2 1 2005

Fingerprint

Bone Marrow Cells
Cornea
Hematopoietic Stem Cells
Corneal Stroma
Bone Marrow
Antigen-Presenting Cells
Bone Marrow Transplantation
Inbred C57BL Mouse
Dendritic Cells
Transgenic Mice
Stem Cells
Transplantation
Immunohistochemistry
Macrophages
Bone and Bones

All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

これを引用

Characterization and distribution of bone marrow-derived cells in mouse cornea. / Nakamura, Takahiro; Ishikawa, Fumihiko; Sonoda, Koh Hei; Hisatomi, Toshio; Qiao, Hong; Yamada, Jun; Fukata, Mitsuhiro; Ishibashi, Tatsuro; Harada, Mine; Kinoshita, Shigeru.

:: Investigative Ophthalmology and Visual Science, 巻 46, 番号 2, 01.02.2005, p. 497-503.

研究成果: ジャーナルへの寄稿記事

Nakamura, Takahiro ; Ishikawa, Fumihiko ; Sonoda, Koh Hei ; Hisatomi, Toshio ; Qiao, Hong ; Yamada, Jun ; Fukata, Mitsuhiro ; Ishibashi, Tatsuro ; Harada, Mine ; Kinoshita, Shigeru. / Characterization and distribution of bone marrow-derived cells in mouse cornea. :: Investigative Ophthalmology and Visual Science. 2005 ; 巻 46, 番号 2. pp. 497-503.
@article{5ac276d1e22b4fcb989ec6bccc2ce94c,
title = "Characterization and distribution of bone marrow-derived cells in mouse cornea",
abstract = "PURPOSE. Bone marrow (BM)- derived stem cells are thought to possess extensive differentiation capacity. The present study was conducted to investigate the characteristics and distribution of these cells in the normal mouse cornea. METHODS. BM cells and BM-derived hematopoietic stem/progenitor cells (HSCs) from enhanced GFP (eGFP) transgenic mice (lin-, Sca-1+) were intravenously transplanted into irradiated wild-type C57BL/6 mice. At 4 to 6 months after transplantation, the mice were killed, and their whole corneas examined by histologic and immunohistochemical methods (CD11c, CD11b, and CD45). RESULTS. At 2 weeks after BM cell transplantation, GFP+ cells gradually migrated into the cornea from the limbal area. At 2 to 6 months, they were distributed over the entire cornea. In cross sections of whole cornea, GFP+ cells comprised 27.3{\%} ± 11.1{\%} (BM) and 24.0{\%} ± 8.01{\%} (HSC) of total cells in the peripheral corneal stroma. In the center of the corneal stroma, GFP+ cells were 7.58{\%} ± 2.63{\%} (BM) and 8.06{\%} ± 1.76{\%} (HSC) of total cells. Immunohistochemistry showed that GFP+ CD11c+, CD11b +, CD11c-, and CD11b- cells occupied the entire corneal stroma. CONCLUSIONS. The present study provides direct evidence of the distribution of BM-derived cells in the mouse cornea. Immunohistochemical study showed that some of these cells are BM-derived antigen-presenting cells such as dendritic cells and macrophages. Some elements of BM-derived cells may continue to exist in the corneal stroma.",
author = "Takahiro Nakamura and Fumihiko Ishikawa and Sonoda, {Koh Hei} and Toshio Hisatomi and Hong Qiao and Jun Yamada and Mitsuhiro Fukata and Tatsuro Ishibashi and Mine Harada and Shigeru Kinoshita",
year = "2005",
month = "2",
day = "1",
doi = "10.1167/iovs.04-1154",
language = "English",
volume = "46",
pages = "497--503",
journal = "Investigative Ophthalmology and Visual Science",
issn = "0146-0404",
publisher = "Association for Research in Vision and Ophthalmology Inc.",
number = "2",

}

TY - JOUR

T1 - Characterization and distribution of bone marrow-derived cells in mouse cornea

AU - Nakamura, Takahiro

AU - Ishikawa, Fumihiko

AU - Sonoda, Koh Hei

AU - Hisatomi, Toshio

AU - Qiao, Hong

AU - Yamada, Jun

AU - Fukata, Mitsuhiro

AU - Ishibashi, Tatsuro

AU - Harada, Mine

AU - Kinoshita, Shigeru

PY - 2005/2/1

Y1 - 2005/2/1

N2 - PURPOSE. Bone marrow (BM)- derived stem cells are thought to possess extensive differentiation capacity. The present study was conducted to investigate the characteristics and distribution of these cells in the normal mouse cornea. METHODS. BM cells and BM-derived hematopoietic stem/progenitor cells (HSCs) from enhanced GFP (eGFP) transgenic mice (lin-, Sca-1+) were intravenously transplanted into irradiated wild-type C57BL/6 mice. At 4 to 6 months after transplantation, the mice were killed, and their whole corneas examined by histologic and immunohistochemical methods (CD11c, CD11b, and CD45). RESULTS. At 2 weeks after BM cell transplantation, GFP+ cells gradually migrated into the cornea from the limbal area. At 2 to 6 months, they were distributed over the entire cornea. In cross sections of whole cornea, GFP+ cells comprised 27.3% ± 11.1% (BM) and 24.0% ± 8.01% (HSC) of total cells in the peripheral corneal stroma. In the center of the corneal stroma, GFP+ cells were 7.58% ± 2.63% (BM) and 8.06% ± 1.76% (HSC) of total cells. Immunohistochemistry showed that GFP+ CD11c+, CD11b +, CD11c-, and CD11b- cells occupied the entire corneal stroma. CONCLUSIONS. The present study provides direct evidence of the distribution of BM-derived cells in the mouse cornea. Immunohistochemical study showed that some of these cells are BM-derived antigen-presenting cells such as dendritic cells and macrophages. Some elements of BM-derived cells may continue to exist in the corneal stroma.

AB - PURPOSE. Bone marrow (BM)- derived stem cells are thought to possess extensive differentiation capacity. The present study was conducted to investigate the characteristics and distribution of these cells in the normal mouse cornea. METHODS. BM cells and BM-derived hematopoietic stem/progenitor cells (HSCs) from enhanced GFP (eGFP) transgenic mice (lin-, Sca-1+) were intravenously transplanted into irradiated wild-type C57BL/6 mice. At 4 to 6 months after transplantation, the mice were killed, and their whole corneas examined by histologic and immunohistochemical methods (CD11c, CD11b, and CD45). RESULTS. At 2 weeks after BM cell transplantation, GFP+ cells gradually migrated into the cornea from the limbal area. At 2 to 6 months, they were distributed over the entire cornea. In cross sections of whole cornea, GFP+ cells comprised 27.3% ± 11.1% (BM) and 24.0% ± 8.01% (HSC) of total cells in the peripheral corneal stroma. In the center of the corneal stroma, GFP+ cells were 7.58% ± 2.63% (BM) and 8.06% ± 1.76% (HSC) of total cells. Immunohistochemistry showed that GFP+ CD11c+, CD11b +, CD11c-, and CD11b- cells occupied the entire corneal stroma. CONCLUSIONS. The present study provides direct evidence of the distribution of BM-derived cells in the mouse cornea. Immunohistochemical study showed that some of these cells are BM-derived antigen-presenting cells such as dendritic cells and macrophages. Some elements of BM-derived cells may continue to exist in the corneal stroma.

UR - http://www.scopus.com/inward/record.url?scp=13944279844&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=13944279844&partnerID=8YFLogxK

U2 - 10.1167/iovs.04-1154

DO - 10.1167/iovs.04-1154

M3 - Article

C2 - 15671274

AN - SCOPUS:13944279844

VL - 46

SP - 497

EP - 503

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

SN - 0146-0404

IS - 2

ER -