Characterization and identification of weissellicin Y and weissellicin M, novel bacteriocins produced by Weissella hellenica QU 13

Yoshimitsu Masuda, Takeshi Zendo, N. Sawa, R. H. Perez, Jiro Nakayama, Kenji Sonomoto

研究成果: ジャーナルへの寄稿記事

33 引用 (Scopus)

抄録

Aims: To identify and characterize novel bacteriocins from Weissella hellenica QU 13. Methods and Results: Weissella hellenica QU 13, isolated from a barrel used to make Japanese pickles, produced two novel bacteriocins termed weissellicin Y and weissellicin M. The primary structures of weissellicins Y and M were determined, and their molecular masses were determined to be 4925·12 and 4968·40Da, respectively. Analysis of the DNA sequence encoding the bacteriocins revealed that they were synthesized and secreted without N-terminal extensions such as leader sequences or sec signal peptides. Weissellicin M showed significantly high and characteristic homology with enterocins L50A and L50B, produced by Enterococcus faecium L50, while weissellicin Y showed no homology with any other known bacteriocins. Both bacteriocins showed broad antimicrobial spectra, with especially high antimicrobial activity against species, which contaminate pickles, such as Bacillus coagulans, and weissellicin M showed relatively higher activity than weissellicin Y. Furthermore, the stability of weissellicin M against pH and heat was distinctively higher than that of weissellicin Y. Conclusions: Weissella hellenica QU 13 produced two novel leaderless bacteriocins, weissellicin Y and weissellicin M, and weissellicin M exhibited remarkable potency that could be employed by pickle-producing industry. Significance and Impact of the Study:  This study is the first report, which represents a complete identification and characterization of novel leaderless bacteriocins from Weissella genus.

元の言語英語
ページ(範囲)99-108
ページ数10
ジャーナルJournal of Applied Microbiology
112
発行部数1
DOI
出版物ステータス出版済み - 1 1 2012

Fingerprint

Weissella
Bacteriocins
Enterococcus faecium
Protein Sorting Signals
DNA Sequence Analysis
Industry
Hot Temperature

All Science Journal Classification (ASJC) codes

  • Applied Microbiology and Biotechnology
  • Biotechnology

これを引用

Characterization and identification of weissellicin Y and weissellicin M, novel bacteriocins produced by Weissella hellenica QU 13. / Masuda, Yoshimitsu; Zendo, Takeshi; Sawa, N.; Perez, R. H.; Nakayama, Jiro; Sonomoto, Kenji.

:: Journal of Applied Microbiology, 巻 112, 番号 1, 01.01.2012, p. 99-108.

研究成果: ジャーナルへの寄稿記事

@article{7ead93676d054278a767038754c361d2,
title = "Characterization and identification of weissellicin Y and weissellicin M, novel bacteriocins produced by Weissella hellenica QU 13",
abstract = "Aims: To identify and characterize novel bacteriocins from Weissella hellenica QU 13. Methods and Results: Weissella hellenica QU 13, isolated from a barrel used to make Japanese pickles, produced two novel bacteriocins termed weissellicin Y and weissellicin M. The primary structures of weissellicins Y and M were determined, and their molecular masses were determined to be 4925·12 and 4968·40Da, respectively. Analysis of the DNA sequence encoding the bacteriocins revealed that they were synthesized and secreted without N-terminal extensions such as leader sequences or sec signal peptides. Weissellicin M showed significantly high and characteristic homology with enterocins L50A and L50B, produced by Enterococcus faecium L50, while weissellicin Y showed no homology with any other known bacteriocins. Both bacteriocins showed broad antimicrobial spectra, with especially high antimicrobial activity against species, which contaminate pickles, such as Bacillus coagulans, and weissellicin M showed relatively higher activity than weissellicin Y. Furthermore, the stability of weissellicin M against pH and heat was distinctively higher than that of weissellicin Y. Conclusions: Weissella hellenica QU 13 produced two novel leaderless bacteriocins, weissellicin Y and weissellicin M, and weissellicin M exhibited remarkable potency that could be employed by pickle-producing industry. Significance and Impact of the Study:  This study is the first report, which represents a complete identification and characterization of novel leaderless bacteriocins from Weissella genus.",
author = "Yoshimitsu Masuda and Takeshi Zendo and N. Sawa and Perez, {R. H.} and Jiro Nakayama and Kenji Sonomoto",
year = "2012",
month = "1",
day = "1",
doi = "10.1111/j.1365-2672.2011.05180.x",
language = "English",
volume = "112",
pages = "99--108",
journal = "Journal of Applied Microbiology",
issn = "1364-5072",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Characterization and identification of weissellicin Y and weissellicin M, novel bacteriocins produced by Weissella hellenica QU 13

AU - Masuda, Yoshimitsu

AU - Zendo, Takeshi

AU - Sawa, N.

AU - Perez, R. H.

AU - Nakayama, Jiro

AU - Sonomoto, Kenji

PY - 2012/1/1

Y1 - 2012/1/1

N2 - Aims: To identify and characterize novel bacteriocins from Weissella hellenica QU 13. Methods and Results: Weissella hellenica QU 13, isolated from a barrel used to make Japanese pickles, produced two novel bacteriocins termed weissellicin Y and weissellicin M. The primary structures of weissellicins Y and M were determined, and their molecular masses were determined to be 4925·12 and 4968·40Da, respectively. Analysis of the DNA sequence encoding the bacteriocins revealed that they were synthesized and secreted without N-terminal extensions such as leader sequences or sec signal peptides. Weissellicin M showed significantly high and characteristic homology with enterocins L50A and L50B, produced by Enterococcus faecium L50, while weissellicin Y showed no homology with any other known bacteriocins. Both bacteriocins showed broad antimicrobial spectra, with especially high antimicrobial activity against species, which contaminate pickles, such as Bacillus coagulans, and weissellicin M showed relatively higher activity than weissellicin Y. Furthermore, the stability of weissellicin M against pH and heat was distinctively higher than that of weissellicin Y. Conclusions: Weissella hellenica QU 13 produced two novel leaderless bacteriocins, weissellicin Y and weissellicin M, and weissellicin M exhibited remarkable potency that could be employed by pickle-producing industry. Significance and Impact of the Study:  This study is the first report, which represents a complete identification and characterization of novel leaderless bacteriocins from Weissella genus.

AB - Aims: To identify and characterize novel bacteriocins from Weissella hellenica QU 13. Methods and Results: Weissella hellenica QU 13, isolated from a barrel used to make Japanese pickles, produced two novel bacteriocins termed weissellicin Y and weissellicin M. The primary structures of weissellicins Y and M were determined, and their molecular masses were determined to be 4925·12 and 4968·40Da, respectively. Analysis of the DNA sequence encoding the bacteriocins revealed that they were synthesized and secreted without N-terminal extensions such as leader sequences or sec signal peptides. Weissellicin M showed significantly high and characteristic homology with enterocins L50A and L50B, produced by Enterococcus faecium L50, while weissellicin Y showed no homology with any other known bacteriocins. Both bacteriocins showed broad antimicrobial spectra, with especially high antimicrobial activity against species, which contaminate pickles, such as Bacillus coagulans, and weissellicin M showed relatively higher activity than weissellicin Y. Furthermore, the stability of weissellicin M against pH and heat was distinctively higher than that of weissellicin Y. Conclusions: Weissella hellenica QU 13 produced two novel leaderless bacteriocins, weissellicin Y and weissellicin M, and weissellicin M exhibited remarkable potency that could be employed by pickle-producing industry. Significance and Impact of the Study:  This study is the first report, which represents a complete identification and characterization of novel leaderless bacteriocins from Weissella genus.

UR - http://www.scopus.com/inward/record.url?scp=83555177236&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=83555177236&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2672.2011.05180.x

DO - 10.1111/j.1365-2672.2011.05180.x

M3 - Article

VL - 112

SP - 99

EP - 108

JO - Journal of Applied Microbiology

JF - Journal of Applied Microbiology

SN - 1364-5072

IS - 1

ER -