Characterization and recombinant protein expression of ferritin light chain homologue in the silkworm, Bombyx mori

研究成果: Contribution to journalArticle査読

10 被引用数 (Scopus)

抄録

The silkworm genome encodes three iron storage proteins or ferritins, Fer1HCH, Fer2LCH, and Fer3HCH. Probing our EST library constructed from 1-day-old silkworm eggs revealed only Fer2LCH mRNA, which encoded for a protein with a predicted putative N-glycosylation site. Developmental and tissue expression analyses during embryogenesis revealed that Fer2LCH mRNA was abundant from 6 h to 6 days after oviposition. Transcriptional expression of Fer2LCH during the postembryonic stage is also high in the larval fat body and mid-gut, and then is upregulated in all pupal tissues tested. We found that Fer2LCH mRNA contains an iron-responsive element, suggesting this ferritin subunit is subject to translational control. Although ferritin expression has been shown to increase following immune challenge in other insects, the levels of Fer2LCH mRNA were not significantly induced following viral or bacterial infection of Bombyx mori. Using a baculovirus expression system we expressed recombinant BmFer2LCH protein, which was detectable in the cytoplasmic fraction, likely in a compartment of the secretory pathway, and was shown to undergo posttranslational modifications including N-glycosylation. In particular, rBmFer2LCH carbohydrate chains were composed of mannose and GlcNAc. We suggest that Fer2LCH is important for iron homeostasis and maintaining normal organ function in silkworms.

本文言語英語
ページ(範囲)135-146
ページ数12
ジャーナルInsect Science
21
2
DOI
出版ステータス出版済み - 4 2014

All Science Journal Classification (ASJC) codes

  • 生態、進化、行動および分類学
  • 農業および作物学
  • 生化学、遺伝学、分子生物学(全般)
  • 昆虫科学

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