Characterization by EPR spectroscopy of cytochrome b-562 isolated from the cytochrome b-c1 complex of Rhodopseudomonas sphaeroides R-26

Koh Iba, Ken Ichiro Takamiya, Shigeru Itoh

研究成果: Contribution to journalArticle査読

4 被引用数 (Scopus)

抄録

The EPR spectra of cytochrome b-562 isolated from the cytochrome b-c1 complex of Rhodopseudomonas sphaeroides were measured at liquid helium temperature. The purified cytochrome b-562 gives a high spin signal at g=6.0. Anaerobic titration of this signal confirmed the presence of two redox components with Em=40 and -110 mV at pH 7.5. These values are consistent with the published ones, Em= 55 and -100 mV at pH 7.0, that were optically estimated for the same type of preparation (Iba et al. (1985) FEBS Lett. 183, 151-154). The power saturation behavior of the g= 6.0 signal at different redox potentials indicated a direct spin-spin interaction between these two redox centers.

本文言語英語
ページ(範囲)1477-1480
ページ数4
ジャーナルJournal of biochemistry
100
6
DOI
出版ステータス出版済み - 10 1 1986

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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