Characterization of 1-methyladenine binding in starfish oocyte cortices

M. Yoshikuni, K. Ishikawa, M. Isobe, T. Goto, Y. Nagahama

研究成果: Contribution to journalArticle査読

48 被引用数 (Scopus)

抄録

1-Methyladenine (1MeAde) is the naturally occurring maturation-inducing hormone of starfish oocytes. We have prepared a biologically active [3H]1MeAde of high purity and relatively high specific radioactivity. This ligand binds to cortices isolated from full-grown prophase-arrested oocytes of the starfish Asterina pectinifera. The binding of [3H]1MeAde to cortices was rapid and reached equilibrium in ~15 min. This is in excellent agreement with the hormone-dependent period required for the induction of oocyte maturation. Binding was maximal between pH 6.4 and 8.0 and diminished sharply above and below this range. Analysis of Scatchard plots of the equilibrium binding of [3H]1MeAde to cortices indicates the existence of a single class of binding site with a dissociation constant of 0.3 μM and a binding capacity of 0.02 fmol per cortex. Whereas biologically active analogs (1-benzyladenine, 1-ethyladenine) inhibited the specific binding of [3H]1MeAde by cortices, biologically inactive analogs (2-methyladenine, 3-methyladenine, 1,9-dimethyladenine, and 1-methylhypoxanthine) did not. These results suggest that the 1MeAde binding characterized herein is necessary for the maturational action of 1MeAde on starfish oocytes.

本文言語英語
ページ(範囲)1874-1877
ページ数4
ジャーナルProceedings of the National Academy of Sciences of the United States of America
85
6
DOI
出版ステータス出版済み - 1988
外部発表はい

All Science Journal Classification (ASJC) codes

  • 一般

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