Characterization of a mouse gene (Fbxw6) that encodes a homologue of Caenorhabditis elegans SEL-10

Shun Ichiro Maruyama, Shigetsugu Hatakeyama, Keiko Nakayama, Noriko Ishida, Koichi Kawakami, Kei Ichi Nakayama

研究成果: ジャーナルへの寄稿記事

21 引用 (Scopus)

抄録

The SCF complex is a type of ubiquitin ligase that consists of the invariable components SKP1, CUL1, and RBX1 as well as a variable component, known as an F-box protein, that is the main determinant of substrate specificity. The Caenorhabditis elegans F-box- and WD40-repeat-containing protein SEL-10 functionally and physically associates with LIN-12 and SEL-12, orthologues of mammalian Notch and presenilin, respectively. We have now identified a gene (which we call Fbxw6) that encodes a mouse homologue (F-box-WD40 repeat protein 6, or FBW6) of SEL-10 and is expressed mainly in brain, heart, and testis. Co-immuno-precipitation analysis showed that FBW6 interacts with SKP1 and CUL1, indicating that these three proteins form an SCF complex. Comparison of the genomic organization of Fbxw6, which is located on mouse chromosome 3.3E3, with that of mouse Fbxw1, Fbxw2, and Fbxw4 showed only a low level of similarity, indicating that these genes diverged relatively early and thereafter evolved independently.

元の言語英語
ページ(範囲)214-222
ページ数9
ジャーナルGenomics
78
発行部数3
DOI
出版物ステータス出版済み - 9 15 2001

Fingerprint

Caenorhabditis elegans
F-Box Proteins
Presenilins
Genes
Proteins
Ligases
Ubiquitin
Substrate Specificity
Testis
Chromosomes
Brain
WD40 Repeats

All Science Journal Classification (ASJC) codes

  • Genetics

これを引用

Characterization of a mouse gene (Fbxw6) that encodes a homologue of Caenorhabditis elegans SEL-10. / Maruyama, Shun Ichiro; Hatakeyama, Shigetsugu; Nakayama, Keiko; Ishida, Noriko; Kawakami, Koichi; Nakayama, Kei Ichi.

:: Genomics, 巻 78, 番号 3, 15.09.2001, p. 214-222.

研究成果: ジャーナルへの寄稿記事

Maruyama, SI, Hatakeyama, S, Nakayama, K, Ishida, N, Kawakami, K & Nakayama, KI 2001, 'Characterization of a mouse gene (Fbxw6) that encodes a homologue of Caenorhabditis elegans SEL-10', Genomics, 巻. 78, 番号 3, pp. 214-222. https://doi.org/10.1006/geno.2001.6658
Maruyama, Shun Ichiro ; Hatakeyama, Shigetsugu ; Nakayama, Keiko ; Ishida, Noriko ; Kawakami, Koichi ; Nakayama, Kei Ichi. / Characterization of a mouse gene (Fbxw6) that encodes a homologue of Caenorhabditis elegans SEL-10. :: Genomics. 2001 ; 巻 78, 番号 3. pp. 214-222.
@article{b73842d582a840af90af1155dfd4f2f9,
title = "Characterization of a mouse gene (Fbxw6) that encodes a homologue of Caenorhabditis elegans SEL-10",
abstract = "The SCF complex is a type of ubiquitin ligase that consists of the invariable components SKP1, CUL1, and RBX1 as well as a variable component, known as an F-box protein, that is the main determinant of substrate specificity. The Caenorhabditis elegans F-box- and WD40-repeat-containing protein SEL-10 functionally and physically associates with LIN-12 and SEL-12, orthologues of mammalian Notch and presenilin, respectively. We have now identified a gene (which we call Fbxw6) that encodes a mouse homologue (F-box-WD40 repeat protein 6, or FBW6) of SEL-10 and is expressed mainly in brain, heart, and testis. Co-immuno-precipitation analysis showed that FBW6 interacts with SKP1 and CUL1, indicating that these three proteins form an SCF complex. Comparison of the genomic organization of Fbxw6, which is located on mouse chromosome 3.3E3, with that of mouse Fbxw1, Fbxw2, and Fbxw4 showed only a low level of similarity, indicating that these genes diverged relatively early and thereafter evolved independently.",
author = "Maruyama, {Shun Ichiro} and Shigetsugu Hatakeyama and Keiko Nakayama and Noriko Ishida and Koichi Kawakami and Nakayama, {Kei Ichi}",
year = "2001",
month = "9",
day = "15",
doi = "10.1006/geno.2001.6658",
language = "English",
volume = "78",
pages = "214--222",
journal = "Genomics",
issn = "0888-7543",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Characterization of a mouse gene (Fbxw6) that encodes a homologue of Caenorhabditis elegans SEL-10

AU - Maruyama, Shun Ichiro

AU - Hatakeyama, Shigetsugu

AU - Nakayama, Keiko

AU - Ishida, Noriko

AU - Kawakami, Koichi

AU - Nakayama, Kei Ichi

PY - 2001/9/15

Y1 - 2001/9/15

N2 - The SCF complex is a type of ubiquitin ligase that consists of the invariable components SKP1, CUL1, and RBX1 as well as a variable component, known as an F-box protein, that is the main determinant of substrate specificity. The Caenorhabditis elegans F-box- and WD40-repeat-containing protein SEL-10 functionally and physically associates with LIN-12 and SEL-12, orthologues of mammalian Notch and presenilin, respectively. We have now identified a gene (which we call Fbxw6) that encodes a mouse homologue (F-box-WD40 repeat protein 6, or FBW6) of SEL-10 and is expressed mainly in brain, heart, and testis. Co-immuno-precipitation analysis showed that FBW6 interacts with SKP1 and CUL1, indicating that these three proteins form an SCF complex. Comparison of the genomic organization of Fbxw6, which is located on mouse chromosome 3.3E3, with that of mouse Fbxw1, Fbxw2, and Fbxw4 showed only a low level of similarity, indicating that these genes diverged relatively early and thereafter evolved independently.

AB - The SCF complex is a type of ubiquitin ligase that consists of the invariable components SKP1, CUL1, and RBX1 as well as a variable component, known as an F-box protein, that is the main determinant of substrate specificity. The Caenorhabditis elegans F-box- and WD40-repeat-containing protein SEL-10 functionally and physically associates with LIN-12 and SEL-12, orthologues of mammalian Notch and presenilin, respectively. We have now identified a gene (which we call Fbxw6) that encodes a mouse homologue (F-box-WD40 repeat protein 6, or FBW6) of SEL-10 and is expressed mainly in brain, heart, and testis. Co-immuno-precipitation analysis showed that FBW6 interacts with SKP1 and CUL1, indicating that these three proteins form an SCF complex. Comparison of the genomic organization of Fbxw6, which is located on mouse chromosome 3.3E3, with that of mouse Fbxw1, Fbxw2, and Fbxw4 showed only a low level of similarity, indicating that these genes diverged relatively early and thereafter evolved independently.

UR - http://www.scopus.com/inward/record.url?scp=0035885864&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035885864&partnerID=8YFLogxK

U2 - 10.1006/geno.2001.6658

DO - 10.1006/geno.2001.6658

M3 - Article

C2 - 11735228

AN - SCOPUS:0035885864

VL - 78

SP - 214

EP - 222

JO - Genomics

JF - Genomics

SN - 0888-7543

IS - 3

ER -