Characterization of a PA14 domain-containing galactofuranose-specific β-D-galactofuranosidase from Streptomyces sp.

Emiko Matsunaga, Yujiro Higuchi, Kazuki Mori, Nao Yairo, Saki Toyota, Takuji Oka, Kosuke Tashiro, Kaoru Takegawa

研究成果: ジャーナルへの寄稿学術誌査読

9 被引用数 (Scopus)


As a constituent of polysaccharides and glycoconjugates, β-D-galactofuranose (Galf) exists in several pathogenic microorganisms. Although we recently identified a β-D-galactofuranosidase (Galf-ase) gene, ORF1110, in the Streptomyces strain JHA19, very little is known about the Galf-ase gene. Here, we characterized a strain, named JHA26, in the culture supernatant of which exhibited Galf-ase activity for 4-nitrophenyl β-D-galactofuranoside (pNP-β-D-Galf) as a substrate. Draft genome sequencing of the JHA26 strain revealed a putative gene, termed ORF0643, that encodes Galf-ase containing a PA14 domain, which is thought to function in substrate recognition. The recombinant protein expressed in Escherichia coli showed the Galf-specific Galf-ase activity and also released galactose residue of the polysaccharide galactomannan prepared from Aspergillus fumigatus, suggesting that this enzyme is an exo-type Galf-ase. BLAST searches using the amino acid sequences of ORF0643 and ORF1110 Galf-ases revealed two types of Galf-ases in Acti-nobacteria, suggesting that Galf-specific Galf-ases may exhibit discrete substrate specificities.

ジャーナルBioscience, Biotechnology and Biochemistry
出版ステータス出版済み - 2017

!!!All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 分析化学
  • 生化学
  • 応用微生物学とバイオテクノロジー
  • 分子生物学
  • 有機化学


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