Characterization of a PA14 domain-containing galactofuranose-specific β-D-galactofuranosidase from Streptomyces sp.

Emiko Matsunaga, Yujiro Higuchi, Kazuki Mori, Nao Yairo, Saki Toyota, Takuji Oka, Kosuke Tashiro, Kaoru Takegawa

研究成果: ジャーナルへの寄稿記事

5 引用 (Scopus)

抄録

As a constituent of polysaccharides and glycoconjugates, β-D-galactofuranose (Galf) exists in several pathogenic microorganisms. Although we recently identified a β-D-galactofuranosidase (Galf-ase) gene, ORF1110, in the Streptomyces strain JHA19, very little is known about the Galf-ase gene. Here, we characterized a strain, named JHA26, in the culture supernatant of which exhibited Galf-ase activity for 4-nitrophenyl β-D-galactofuranoside (pNP-β-D-Galf) as a substrate. Draft genome sequencing of the JHA26 strain revealed a putative gene, termed ORF0643, that encodes Galf-ase containing a PA14 domain, which is thought to function in substrate recognition. The recombinant protein expressed in Escherichia coli showed the Galf-specific Galf-ase activity and also released galactose residue of the polysaccharide galactomannan prepared from Aspergillus fumigatus, suggesting that this enzyme is an exo-type Galf-ase. BLAST searches using the amino acid sequences of ORF0643 and ORF1110 Galf-ases revealed two types of Galf-ases in Acti-nobacteria, suggesting that Galf-specific Galf-ases may exhibit discrete substrate specificities.

元の言語英語
ページ(範囲)1314-1319
ページ数6
ジャーナルBioscience, Biotechnology and Biochemistry
81
発行部数7
DOI
出版物ステータス出版済み - 1 1 2017

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Streptomyces
Genes
Polysaccharides
Glycoconjugates
Aspergillus fumigatus
Substrates
Substrate Specificity
Galactose
Recombinant Proteins
Aspergillus
Amino Acid Sequence
Genome
Microorganisms
Escherichia coli
Enzymes
Amino Acids
5-O-beta-galactofuranosyl-galactofuranose

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

これを引用

Characterization of a PA14 domain-containing galactofuranose-specific β-D-galactofuranosidase from Streptomyces sp. / Matsunaga, Emiko; Higuchi, Yujiro; Mori, Kazuki; Yairo, Nao; Toyota, Saki; Oka, Takuji; Tashiro, Kosuke; Takegawa, Kaoru.

:: Bioscience, Biotechnology and Biochemistry, 巻 81, 番号 7, 01.01.2017, p. 1314-1319.

研究成果: ジャーナルへの寄稿記事

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