Characterization of a rice nuclear-encoded plastid RNA polymerase gene OsRpoTp

Kensuke Kusumi, Asanori Yara, Naoko Mitsui, Yuzuru Tozawa, Koh Iba

研究成果: ジャーナルへの寄稿学術誌査読

63 被引用数 (Scopus)

抄録

We isolated and characterized two rice genes, OsRpoTp and OsRpoTm, that encode putative phage-type RNA polymerases. Predicted amino acid sequences showed high homology of these genes to known RpoT genes. A transient expression assay using green fluorescent protein indicated that the encoded proteins were localized to plastids and mitochondria, respectively. We demonstrated by reverse transcription-PCR experiments and immunoblot analysis that OsRpoTp expression occurred at an early stage of leaf development, prior to the transcript accumulation of the genes that were transcribed by the nuclear-encoded plastid RNA polymerase (NEP). Expression analyses of the chloroplast-deficient rice mutant, virescent-1, showed a discrepancy between OsRpoTp protein accumulation and the level of transcripts of NEP-transcribed genes. Our results suggest that NEP activation is regulated by a process after transcription, and is affected by the developmental state of chloroplast biogenesis.

本文言語英語
ページ(範囲)1194-1201
ページ数8
ジャーナルPlant and Cell Physiology
45
9
DOI
出版ステータス出版済み - 9月 2004

!!!All Science Journal Classification (ASJC) codes

  • 生理学
  • 植物科学
  • 細胞生物学

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