Characterization of an omega-class glutathione S-transferase in the stress response of the silkmoth

Kohji Yamamoto, S. Teshiba, Y. Shigeoka, Y. Aso, Yutaka Banno, T. Fujiki, Yoshinori Katakura

研究成果: ジャーナルへの寄稿記事

22 引用 (Scopus)

抄録

The glutathione S-transferase (GST) superfamily is involved in detoxification of various xenobiotics. Using real-time PCR, mRNA encoding an omega-class GST of Bombyx mori (bmGSTO) was shown to be induced after exposure to various environmental stresses. A soluble form of recombinant protein (rbmGSTO) was functionally overexpressed in Escherichia coli cells and purified to homogeneity.Cys 38 and Pro 39 were found to be highly conserved in omega-class GSTs, and their roles were investigated by site-directed mutagenesis/kinetic analysis. Mutations of Cys 38 and Pro 39 residues affected the catalytic efficiency of enzymes, indicating that the presence of Cys 38 and Pro 39 residues is important for bmGSTO activity. Thus, bmGSTO could contribute to increasing the environmental stress resistance of lepidopteran insects.

元の言語英語
ページ(範囲)379-386
ページ数8
ジャーナルInsect Molecular Biology
20
発行部数3
DOI
出版物ステータス出版済み - 6 1 2011

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Bombyx
Glutathione Transferase
glutathione transferase
stress response
site-directed mutagenesis
Xenobiotics
xenobiotics
Bombyx mori
Site-Directed Mutagenesis
catalytic activity
Recombinant Proteins
recombinant proteins
stress tolerance
Insects
Real-Time Polymerase Chain Reaction
quantitative polymerase chain reaction
Lepidoptera
Escherichia coli
mutation
kinetics

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Insect Science

これを引用

Characterization of an omega-class glutathione S-transferase in the stress response of the silkmoth. / Yamamoto, Kohji; Teshiba, S.; Shigeoka, Y.; Aso, Y.; Banno, Yutaka; Fujiki, T.; Katakura, Yoshinori.

:: Insect Molecular Biology, 巻 20, 番号 3, 01.06.2011, p. 379-386.

研究成果: ジャーナルへの寄稿記事

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AU - Fujiki, T.

AU - Katakura, Yoshinori

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