Characterization of Armitage and Yb containing granules and their relationship to nuage in ovary-derived cultured silkworm cell

Anandrao Ashok Patil, Tsuneyuki Tatsuke, Hiroaki Mon, Man Lee, Daisuke Morokuma, Masato Hino, Takahiro Kusakabe

研究成果: ジャーナルへの寄稿記事

2 引用 (Scopus)

抄録

PIWI-interacting RNAs (piRNAs) are a class of endogenous small non-coding RNAs, which are mostly 24–32 nucleotides in length and interact specifically with PIWI subfamily of argonaute proteins. Despite the significant research progress in germ line piRNA pathway, its role in somatic cell is not well known. In Drosophila ovarian somatic cell, maturation of primary piRNA and its loading onto Piwi occurs at perinuclear Yb body. The Armitage (Armi) and Yb proteins are the major components of Yb body and specially expressed in ovarian somatic cell. Based on the reports, here we studied the BmArmi and BmYb in Bombyx mori ovary-derived BmN4 cells expressing BmVasa. In this study, we show that BmArmi and BmYb co-localized with BmVasa at nuage. The helicase domains of BmArmi and BmYb are important for nuage localization. Moreover, RNAi of piRNA components reveal that BmArmi depend on BmAgo3 for nuage localization, and BmArmi and BmYb form cytoplasmic granules independently in the absence of BmVasa. Our results provide evidence that the BmArmi and BmYb coexist with BmVasa and play an important role in perinuclear nuage granules formation in ovary-derived BmN4 cell.

元の言語英語
ページ(範囲)134-140
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
490
発行部数2
DOI
出版物ステータス出版済み - 8 19 2017

Fingerprint

Bombyx
Small Interfering RNA
Ovary
Cultured Cells
Argonaute Proteins
Small Untranslated RNA
Cytoplasmic Granules
Nucleotides
Cells
RNA
RNA Interference
Germ Cells
Drosophila
Proteins
Research

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

Characterization of Armitage and Yb containing granules and their relationship to nuage in ovary-derived cultured silkworm cell. / Patil, Anandrao Ashok; Tatsuke, Tsuneyuki; Mon, Hiroaki; Lee, Man; Morokuma, Daisuke; Hino, Masato; Kusakabe, Takahiro.

:: Biochemical and Biophysical Research Communications, 巻 490, 番号 2, 19.08.2017, p. 134-140.

研究成果: ジャーナルへの寄稿記事

Patil, Anandrao Ashok ; Tatsuke, Tsuneyuki ; Mon, Hiroaki ; Lee, Man ; Morokuma, Daisuke ; Hino, Masato ; Kusakabe, Takahiro. / Characterization of Armitage and Yb containing granules and their relationship to nuage in ovary-derived cultured silkworm cell. :: Biochemical and Biophysical Research Communications. 2017 ; 巻 490, 番号 2. pp. 134-140.
@article{57bfdfa93e4f48ed9a8087d3915dc6b7,
title = "Characterization of Armitage and Yb containing granules and their relationship to nuage in ovary-derived cultured silkworm cell",
abstract = "PIWI-interacting RNAs (piRNAs) are a class of endogenous small non-coding RNAs, which are mostly 24–32 nucleotides in length and interact specifically with PIWI subfamily of argonaute proteins. Despite the significant research progress in germ line piRNA pathway, its role in somatic cell is not well known. In Drosophila ovarian somatic cell, maturation of primary piRNA and its loading onto Piwi occurs at perinuclear Yb body. The Armitage (Armi) and Yb proteins are the major components of Yb body and specially expressed in ovarian somatic cell. Based on the reports, here we studied the BmArmi and BmYb in Bombyx mori ovary-derived BmN4 cells expressing BmVasa. In this study, we show that BmArmi and BmYb co-localized with BmVasa at nuage. The helicase domains of BmArmi and BmYb are important for nuage localization. Moreover, RNAi of piRNA components reveal that BmArmi depend on BmAgo3 for nuage localization, and BmArmi and BmYb form cytoplasmic granules independently in the absence of BmVasa. Our results provide evidence that the BmArmi and BmYb coexist with BmVasa and play an important role in perinuclear nuage granules formation in ovary-derived BmN4 cell.",
author = "Patil, {Anandrao Ashok} and Tsuneyuki Tatsuke and Hiroaki Mon and Man Lee and Daisuke Morokuma and Masato Hino and Takahiro Kusakabe",
year = "2017",
month = "8",
day = "19",
doi = "10.1016/j.bbrc.2017.06.008",
language = "English",
volume = "490",
pages = "134--140",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Characterization of Armitage and Yb containing granules and their relationship to nuage in ovary-derived cultured silkworm cell

AU - Patil, Anandrao Ashok

AU - Tatsuke, Tsuneyuki

AU - Mon, Hiroaki

AU - Lee, Man

AU - Morokuma, Daisuke

AU - Hino, Masato

AU - Kusakabe, Takahiro

PY - 2017/8/19

Y1 - 2017/8/19

N2 - PIWI-interacting RNAs (piRNAs) are a class of endogenous small non-coding RNAs, which are mostly 24–32 nucleotides in length and interact specifically with PIWI subfamily of argonaute proteins. Despite the significant research progress in germ line piRNA pathway, its role in somatic cell is not well known. In Drosophila ovarian somatic cell, maturation of primary piRNA and its loading onto Piwi occurs at perinuclear Yb body. The Armitage (Armi) and Yb proteins are the major components of Yb body and specially expressed in ovarian somatic cell. Based on the reports, here we studied the BmArmi and BmYb in Bombyx mori ovary-derived BmN4 cells expressing BmVasa. In this study, we show that BmArmi and BmYb co-localized with BmVasa at nuage. The helicase domains of BmArmi and BmYb are important for nuage localization. Moreover, RNAi of piRNA components reveal that BmArmi depend on BmAgo3 for nuage localization, and BmArmi and BmYb form cytoplasmic granules independently in the absence of BmVasa. Our results provide evidence that the BmArmi and BmYb coexist with BmVasa and play an important role in perinuclear nuage granules formation in ovary-derived BmN4 cell.

AB - PIWI-interacting RNAs (piRNAs) are a class of endogenous small non-coding RNAs, which are mostly 24–32 nucleotides in length and interact specifically with PIWI subfamily of argonaute proteins. Despite the significant research progress in germ line piRNA pathway, its role in somatic cell is not well known. In Drosophila ovarian somatic cell, maturation of primary piRNA and its loading onto Piwi occurs at perinuclear Yb body. The Armitage (Armi) and Yb proteins are the major components of Yb body and specially expressed in ovarian somatic cell. Based on the reports, here we studied the BmArmi and BmYb in Bombyx mori ovary-derived BmN4 cells expressing BmVasa. In this study, we show that BmArmi and BmYb co-localized with BmVasa at nuage. The helicase domains of BmArmi and BmYb are important for nuage localization. Moreover, RNAi of piRNA components reveal that BmArmi depend on BmAgo3 for nuage localization, and BmArmi and BmYb form cytoplasmic granules independently in the absence of BmVasa. Our results provide evidence that the BmArmi and BmYb coexist with BmVasa and play an important role in perinuclear nuage granules formation in ovary-derived BmN4 cell.

UR - http://www.scopus.com/inward/record.url?scp=85020687273&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85020687273&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2017.06.008

DO - 10.1016/j.bbrc.2017.06.008

M3 - Article

C2 - 28595904

AN - SCOPUS:85020687273

VL - 490

SP - 134

EP - 140

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -