Characterization of bone marrow derived mesenchymal stem cells in suspension

Kentaro Akiyama, Yong Ouk You, Takayoshi Yamaza, Chider Chen, Liang Tang, Yan Jin, Xiao Dong Chen, Stan Gronthos, Songtao Shi

研究成果: ジャーナルへの寄稿学術誌査読

63 被引用数 (Scopus)

抄録

Introduction. Bone marrow mesenchymal stem cells (BMMSCs) are a heterogeneous population of postnatal precursor cells with the capacity of adhering to culture dishes generating colony-forming unit-fibroblasts (CFU-F). Here we identify a new subset of BMMSCs that fail to adhere to plastic culture dishes and remain in culture suspension (S-BMMSCs). Methods. To catch S-BMMSCs, we used BMMSCs-produced extracellular cell matrix (ECM)-coated dishes. Isolated S-BMMSCs were analyzed by in vitro stem cell analysis approaches, including flow cytometry, inductive multiple differentiation, western blot and in vivo implantation to assess the bone regeneration ability of S-BMMSCs. Furthermore, we performed systemic S-BMMSCs transplantation to treat systemic lupus erythematosus (SLE)-like MRL/lpr mice. Results: S-BMMSCs are capable of adhering to ECM-coated dishes and showing mesenchymal stem cell characteristics with distinction from hematopoietic cells as evidenced by co-expression of CD73 or Oct-4 with CD34, forming a single colony cluster on ECM, and failure to differentiate into hematopoietic cell lineage. Moreover, we found that culture-expanded S-BMMSCs exhibited significantly increased immunomodulatory capacities in vitro and an efficacious treatment for SLE-like MRL/lpr mice by rebalancing regulatory T cells (Tregs) and T helper 17 cells (Th17) through high NO production. Conclusions: These data suggest that it is feasible to improve immunotherapy by identifying a new subset BMMSCs.

本文言語英語
論文番号40
ジャーナルStem Cell Research and Therapy
3
5
DOI
出版ステータス出版済み - 2012

!!!All Science Journal Classification (ASJC) codes

  • 医学(その他)
  • 分子医療
  • 生化学、遺伝学、分子生物学(その他)
  • 細胞生物学

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