TY - JOUR
T1 - Characterization of carbonate apatite derived from chicken bone and its in-vitro evaluation using MC3T3-E1 cells
AU - Tram, Nguyen Xuan Thanh
AU - Ishikawa, Kunio
AU - Minh, Tran Hoang
AU - Benson, David
AU - Tsuru, Kanji
N1 - Publisher Copyright:
© 2021 The Author(s).
PY - 2021/2
Y1 - 2021/2
N2 - Chicken bone by-product has the potential to be utilized as a source of carbonate apatite (CO3Ap) for the preparation of bone grafts. In this study, the raw bones were cleaned with NaCl and then immersed inNaOHsolution at 80 °C. After chemical treatment, the as-prepared powder was calcined in a range of 300 °C-900 °C. The results showed that calcination was useful for the complete elimination of organic residues; but, it led to increasing of crystal size, coarsening of particles, and the loss of the carbonate groups in the apatite structure. In vitro evaluation showed thatCO3Ap prepared at the optimum temperature, 500 °C, exhibited a great biological response; such as good cell attachment, cell viability, and cell morphology. This research suggests a promising biomaterial for the fabrication of bone substitutes and could offer a solution for recycling and adding value to food waste.
AB - Chicken bone by-product has the potential to be utilized as a source of carbonate apatite (CO3Ap) for the preparation of bone grafts. In this study, the raw bones were cleaned with NaCl and then immersed inNaOHsolution at 80 °C. After chemical treatment, the as-prepared powder was calcined in a range of 300 °C-900 °C. The results showed that calcination was useful for the complete elimination of organic residues; but, it led to increasing of crystal size, coarsening of particles, and the loss of the carbonate groups in the apatite structure. In vitro evaluation showed thatCO3Ap prepared at the optimum temperature, 500 °C, exhibited a great biological response; such as good cell attachment, cell viability, and cell morphology. This research suggests a promising biomaterial for the fabrication of bone substitutes and could offer a solution for recycling and adding value to food waste.
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U2 - 10.1088/2053-1591/abe018
DO - 10.1088/2053-1591/abe018
M3 - Article
AN - SCOPUS:85101417484
VL - 8
JO - Materials Research Express
JF - Materials Research Express
SN - 2053-1591
IS - 2
M1 - 025401
ER -