Characterization of CD4- CD8- CD3+ T-cell receptor-αβ+ T cells in murine cytomegalovirus infection

M. S. Hossain, H. Takimoto, T. Ninomiya, H. Yoshida, K. Kishihara, G. Matsuzaki, G. Kimura, K. Nomoto

研究成果: ジャーナルへの寄稿記事

14 引用 (Scopus)

抄録

In this study, we have investigated that after the intraperitoneal infection with murine cytomegalovirus (MCMV), the CD3+ CD4-CD8-(double negative; DN) T-cell receptor (TCR)αβ+ T cells increased in peritoneal cavity, liver and spleen in both resistant C57BL/6 and susceptible BALB/c mice. The total cellular population of these cells showed peak levels around day 5 after infection in all the three investigated organs and the following phenotypical and functional characteristics emerged. The peritoneal DN TCRαβ+ T cells expressed highly skewed TCRVβ8 on day 5 after infection compared with the uninfected mice, but those in spleen and liver showed moderate and low skewed TCRVβ8, respectively. The percentages of NK1.1+ DN TCRαβ+ T cells gradually decreased as did modulation of some of their activation markers consistent with an activated cell phenotype. The peritoneal DN TCRαβ+ T cells on day 5 after infection expressed the genes of interferon-γ (IFN-γ), tumour necrosis factor-α, Eta-1 (early T-cell activation-1) and MCP-1 (monocyte chemoattractant protein 1) but lacked expression of interleukin-4 (IL-4). After in vitro stimulation with phorbol 12-myristate 13-acetate and calcium ionophore in the presence of Brefeldin A, higher frequencies of intracellular IFN-γ+ DN TCRαβ+ T cells were detected in all three investigated organs of infected mice compared with those of uninfected mice. Stimulation of peritoneal DN TCRαβ+ T cells with plate-bound anti-TCRβ monoclonal antibodies showed proliferation and also produced IFN-γ but not IL-4. These results suggest that DN TCRαβ+ T cells were activated and may have an antiviral effect through producing IFN-γ and some macrophage-activating factors during an early phase of MCMV infection.

元の言語英語
ページ(範囲)19-29
ページ数11
ジャーナルImmunology
101
発行部数1
DOI
出版物ステータス出版済み - 1 1 2000

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Muromegalovirus
Cytomegalovirus Infections
T-Cell Antigen Receptor
T-Lymphocytes
Interferons
Infection
Interleukin-4
Spleen
Macrophage-Activating Factors
Brefeldin A
Calcium Ionophores
Chemokine CCL2
Liver
Peritoneal Cavity
Antiviral Agents
Acetates
Tumor Necrosis Factor-alpha
Monoclonal Antibodies
Phenotype

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

これを引用

Hossain, M. S., Takimoto, H., Ninomiya, T., Yoshida, H., Kishihara, K., Matsuzaki, G., ... Nomoto, K. (2000). Characterization of CD4- CD8- CD3+ T-cell receptor-αβ+ T cells in murine cytomegalovirus infection. Immunology, 101(1), 19-29. https://doi.org/10.1046/j.1365-2567.2000.00052.x

Characterization of CD4- CD8- CD3+ T-cell receptor-αβ+ T cells in murine cytomegalovirus infection. / Hossain, M. S.; Takimoto, H.; Ninomiya, T.; Yoshida, H.; Kishihara, K.; Matsuzaki, G.; Kimura, G.; Nomoto, K.

:: Immunology, 巻 101, 番号 1, 01.01.2000, p. 19-29.

研究成果: ジャーナルへの寄稿記事

Hossain, MS, Takimoto, H, Ninomiya, T, Yoshida, H, Kishihara, K, Matsuzaki, G, Kimura, G & Nomoto, K 2000, 'Characterization of CD4- CD8- CD3+ T-cell receptor-αβ+ T cells in murine cytomegalovirus infection', Immunology, 巻. 101, 番号 1, pp. 19-29. https://doi.org/10.1046/j.1365-2567.2000.00052.x
Hossain, M. S. ; Takimoto, H. ; Ninomiya, T. ; Yoshida, H. ; Kishihara, K. ; Matsuzaki, G. ; Kimura, G. ; Nomoto, K. / Characterization of CD4- CD8- CD3+ T-cell receptor-αβ+ T cells in murine cytomegalovirus infection. :: Immunology. 2000 ; 巻 101, 番号 1. pp. 19-29.
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abstract = "In this study, we have investigated that after the intraperitoneal infection with murine cytomegalovirus (MCMV), the CD3+ CD4-CD8-(double negative; DN) T-cell receptor (TCR)αβ+ T cells increased in peritoneal cavity, liver and spleen in both resistant C57BL/6 and susceptible BALB/c mice. The total cellular population of these cells showed peak levels around day 5 after infection in all the three investigated organs and the following phenotypical and functional characteristics emerged. The peritoneal DN TCRαβ+ T cells expressed highly skewed TCRVβ8 on day 5 after infection compared with the uninfected mice, but those in spleen and liver showed moderate and low skewed TCRVβ8, respectively. The percentages of NK1.1+ DN TCRαβ+ T cells gradually decreased as did modulation of some of their activation markers consistent with an activated cell phenotype. The peritoneal DN TCRαβ+ T cells on day 5 after infection expressed the genes of interferon-γ (IFN-γ), tumour necrosis factor-α, Eta-1 (early T-cell activation-1) and MCP-1 (monocyte chemoattractant protein 1) but lacked expression of interleukin-4 (IL-4). After in vitro stimulation with phorbol 12-myristate 13-acetate and calcium ionophore in the presence of Brefeldin A, higher frequencies of intracellular IFN-γ+ DN TCRαβ+ T cells were detected in all three investigated organs of infected mice compared with those of uninfected mice. Stimulation of peritoneal DN TCRαβ+ T cells with plate-bound anti-TCRβ monoclonal antibodies showed proliferation and also produced IFN-γ but not IL-4. These results suggest that DN TCRαβ+ T cells were activated and may have an antiviral effect through producing IFN-γ and some macrophage-activating factors during an early phase of MCMV infection.",
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AU - Takimoto, H.

AU - Ninomiya, T.

AU - Yoshida, H.

AU - Kishihara, K.

AU - Matsuzaki, G.

AU - Kimura, G.

AU - Nomoto, K.

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