Characterization of Haemaphysalis longicornis recombinant cement-like antigens and preliminary study of their vaccination effects

Thasaneeya Harnnoi, Songwut Watchabunsook, Takeshi Sakaguchi, Xuenan Xuan, Kozo Fujisaki

研究成果: ジャーナルへの寄稿記事

抄録

Two genes encoding immunodominant antigens, hlim2 and hlim3, were obtained from a salivary gland cDNA library of the hard tick, Haemaphysalis longicornis. The recombinant proteins were expressed in Escherichia coli as the GST fusion protein and used for immunization. We observed that the attachment rate of nymphal ticks fed on mice immunized with GST-hlim3 was significantly lower than that in the control group during the initial days of feeding. However, immunization with GST-hlim3 did not affect the engorgement rate of the ticks. In sharp contrast, GST-hlim2 did not influence the attachment rate and feeding period of ticks but had a significant reduction in the engorgement body weight. These data highlight the suitability of the 2 recombinant cement-like proteins for use in a cocktail vaccine.

元の言語英語
ページ(範囲)1289-95
ページ数7
ジャーナルJournal of Veterinary Medical Science
68
発行部数12
出版物ステータス出版済み - 12 2006

Fingerprint

Haemaphysalis longicornis
Ticks
cement
ticks
engorgement
Vaccination
vaccination
antigens
Antigens
Immunization
immunization
Ixodidae
Immunodominant Epitopes
salivary glands
Salivary Glands
Gene Library
Recombinant Proteins
recombinant proteins
cDNA libraries
Proteins

これを引用

Characterization of Haemaphysalis longicornis recombinant cement-like antigens and preliminary study of their vaccination effects. / Harnnoi, Thasaneeya; Watchabunsook, Songwut; Sakaguchi, Takeshi; Xuan, Xuenan; Fujisaki, Kozo.

:: Journal of Veterinary Medical Science, 巻 68, 番号 12, 12.2006, p. 1289-95.

研究成果: ジャーナルへの寄稿記事

Harnnoi, Thasaneeya ; Watchabunsook, Songwut ; Sakaguchi, Takeshi ; Xuan, Xuenan ; Fujisaki, Kozo. / Characterization of Haemaphysalis longicornis recombinant cement-like antigens and preliminary study of their vaccination effects. :: Journal of Veterinary Medical Science. 2006 ; 巻 68, 番号 12. pp. 1289-95.
@article{2a4118208f6044c5a2e5fa2954fa319b,
title = "Characterization of Haemaphysalis longicornis recombinant cement-like antigens and preliminary study of their vaccination effects",
abstract = "Two genes encoding immunodominant antigens, hlim2 and hlim3, were obtained from a salivary gland cDNA library of the hard tick, Haemaphysalis longicornis. The recombinant proteins were expressed in Escherichia coli as the GST fusion protein and used for immunization. We observed that the attachment rate of nymphal ticks fed on mice immunized with GST-hlim3 was significantly lower than that in the control group during the initial days of feeding. However, immunization with GST-hlim3 did not affect the engorgement rate of the ticks. In sharp contrast, GST-hlim2 did not influence the attachment rate and feeding period of ticks but had a significant reduction in the engorgement body weight. These data highlight the suitability of the 2 recombinant cement-like proteins for use in a cocktail vaccine.",
author = "Thasaneeya Harnnoi and Songwut Watchabunsook and Takeshi Sakaguchi and Xuenan Xuan and Kozo Fujisaki",
year = "2006",
month = "12",
language = "English",
volume = "68",
pages = "1289--95",
journal = "Journal of Veterinary Medical Science",
issn = "0916-7250",
publisher = "Japanese Society of Veterinary Science",
number = "12",

}

TY - JOUR

T1 - Characterization of Haemaphysalis longicornis recombinant cement-like antigens and preliminary study of their vaccination effects

AU - Harnnoi, Thasaneeya

AU - Watchabunsook, Songwut

AU - Sakaguchi, Takeshi

AU - Xuan, Xuenan

AU - Fujisaki, Kozo

PY - 2006/12

Y1 - 2006/12

N2 - Two genes encoding immunodominant antigens, hlim2 and hlim3, were obtained from a salivary gland cDNA library of the hard tick, Haemaphysalis longicornis. The recombinant proteins were expressed in Escherichia coli as the GST fusion protein and used for immunization. We observed that the attachment rate of nymphal ticks fed on mice immunized with GST-hlim3 was significantly lower than that in the control group during the initial days of feeding. However, immunization with GST-hlim3 did not affect the engorgement rate of the ticks. In sharp contrast, GST-hlim2 did not influence the attachment rate and feeding period of ticks but had a significant reduction in the engorgement body weight. These data highlight the suitability of the 2 recombinant cement-like proteins for use in a cocktail vaccine.

AB - Two genes encoding immunodominant antigens, hlim2 and hlim3, were obtained from a salivary gland cDNA library of the hard tick, Haemaphysalis longicornis. The recombinant proteins were expressed in Escherichia coli as the GST fusion protein and used for immunization. We observed that the attachment rate of nymphal ticks fed on mice immunized with GST-hlim3 was significantly lower than that in the control group during the initial days of feeding. However, immunization with GST-hlim3 did not affect the engorgement rate of the ticks. In sharp contrast, GST-hlim2 did not influence the attachment rate and feeding period of ticks but had a significant reduction in the engorgement body weight. These data highlight the suitability of the 2 recombinant cement-like proteins for use in a cocktail vaccine.

M3 - Article

C2 - 17213697

VL - 68

SP - 1289

EP - 1295

JO - Journal of Veterinary Medical Science

JF - Journal of Veterinary Medical Science

SN - 0916-7250

IS - 12

ER -