Characterization of low viscosity polymer solutions for microchip electrophoresis of non-denatured proteins on plastic chips

Takao Yasui, Mohamad Reza Mohamadi, Noritada Kaji, Yukihiro Okamoto, Manabu Tokeshi, Yoshinobu Baba

研究成果: Contribution to journalArticle査読

16 被引用数 (Scopus)

抄録

In this paper, we study characteristics of polymers (methylcellulose, hypromellose ((hydroxypropyl)methyl cellulose), poly(vinylpyrrolidone), and poly(vinyl alcohol)) with different chemical structures for microchip electrophoresis of non-denatured protein samples in a plastic microchip made of poly(methyl methacrylate) (PMMA). Coating efficiency of these polymers for controlling protein adsorption onto the channel surface of the plastic microchip, wettability of the PMMA surface, and electroosmotic flow in the PMMA microchannels in the presence of these polymers were compared. Also relative electrophoretic mobility of protein samples in solutions of these polymers was studied. We showed that when using low polymer concentrations (lower than the polymer entanglement point) where the sieving effect is substantially negligible, the interaction of the samples with the polymer affected the electrophoretic mobility of the samples. This effect can be used for achieving better resolution in microchip electrophoresis of protein samples.

本文言語英語
論文番号044114
ジャーナルBiomicrofluidics
5
4
DOI
出版ステータス出版済み - 11 11 2011
外部発表はい

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Materials Science(all)
  • Genetics
  • Condensed Matter Physics
  • Physical and Theoretical Chemistry

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