Characterization of microsomal alcohol oxygenase catalyzing the oxidation of 7-hydroxy-Δ8-tetrahydrocannabinol to 7-oxo-Δ8- tetrahydrocannabinol in rat liver

Tamihide Matsunaga, Kinya Shibayama, Shinsuke Higuchi, Hiroyuki Tanaka, Kazuhito Watanabe, Ikuo Yamamoto

研究成果: Contribution to journalArticle査読

6 被引用数 (Scopus)

抄録

The formation of 7-oxo-Δ8-tetrahydrocannabinol (7-oxo-Δ8-THC) from 7β-hydroxy-Δ8-THC was found in hepatic microsomes of rats. The activity was stereoselective and about 3-fold higher than that from 7α-hydroxy-Δ8- THC. The oxidative activity of 7α- and 7β-hydroxy-Δ8-THC to 7-oxo-Δ8- THC was significantly higher in male than in female, and significantly enhanced by both dexamethasone and phenobarbital, and then inhibited up to about 20% of the control value by antibody against P450GPF-B, presumably a member of the 3A subfamily, a major enzyme responsible for the formation of 7-oxo-Δ8-THC in guinea pigs. This antibody also inhibited the formation of 7α- and 7β-hydroxy-Δ8-THC, and 7-oxo-Δ8-THC from Δ8-THC by hepatic microsomes of rats. These results indicate that there is a sex-related difference in the oxidation of 7-hydroxy-Δ8-THC to 7-oxo-Δ8-THC and the reaction is mainly catalyzed by P450 enzyme(s) belonging to the 3A subfamily as major enzyme(s) of microsomal alcohol oxygenase in rats.

本文言語英語
ページ(範囲)43-46
ページ数4
ジャーナルBiological and Pharmaceutical Bulletin
23
1
DOI
出版ステータス出版済み - 1 2000

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

フィンガープリント 「Characterization of microsomal alcohol oxygenase catalyzing the oxidation of 7-hydroxy-Δ<sup>8</sup>-tetrahydrocannabinol to 7-oxo-Δ<sup>8</sup>- tetrahydrocannabinol in rat liver」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル