Characterization of N- and O-linked galactosylated oligosaccharides from fission yeast species

Takamasa Fukunaga, Naotaka Tanaka, Toshio Furumoto, Shinichi Nakakita, Takao Ohashi, Yujiro Higuchi, Hiromi Maekawa, Kaoru Takegawa

研究成果: ジャーナルへの寄稿学術誌査読

4 被引用数 (Scopus)


The N- and O-linked oligosaccharides from fission yeast Schizosaccharomyces pombe not only contain large amounts of D-mannose (Man) but also contain large amounts of D-galactose (Gal). Although the galactomannans of S. pombe are mainly composed of α1,2- or α1,3-linked Gals, some of the terminal α1,2-linked Gals are found to be linked to pyruvylated β1,3-linked galactose (PvGal). We have determined the structural characteristics of the N-glycans and O-glycans in three Schizosaccharomyces species (S. japonicus, S. octosporus, and S. cryophilus) using lectin blot, 1H NMR spectroscopy, and size-fractionation high performance liquid chromatography (HPLC), and found that the galactosylation of oligosaccharides was a common feature in fission yeasts. In addition, each of the terminal Galα1,2-, Galβ1,3- and non-substituted Man residues exhibited distinct characteristics. A BLAST search of gene databases in Schizosaccharomyces identified genes homologous to pvg1 encoding pyruvyltransferase of S. pombe. These genes, when expressed in an S. pombe pvg1Δ strains, led to the pyruvylation of non-reducing terminal β-linked Gal, suggesting the biosynthetic pathway of PvGal-containing oligosaccharides is highly conserved in fission yeasts.

ジャーナルJournal of Bioscience and Bioengineering
出版ステータス出版済み - 8月 2020

!!!All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • バイオエンジニアリング
  • 応用微生物学とバイオテクノロジー


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