Characterization of natriuretic peptide receptors in cultured cells

Shin Ichi Suga, Kazuwa Nakao, Masashi Mukoyama, Hiroshi Arai, Kiminori Hosoda, Yoshihiro Ogawa, Hiroo Imura

研究成果: Contribution to journalArticle査読

56 被引用数 (Scopus)

抄録

To elucidate physiological and clinical implications of the natriuretic peptide family, the expression of receptors for natriuretic peptides has been examined in cultured cells (a rat pheochromocytoma cell line [PC12], bovine endothelial cells, rat aortic smooth muscle cells, human mesangial cells, and a porcine kidney epithelial cell line [LLC-PK1]) by Northern blot analysis and cyclic GMP production method for the ANP-A and ANP-B receptors and by Northern blot analysis and binding assay for the clearance receptor. The ANP-A receptor was predominantly expressed in PC12 cells, bovine endothelial cells, and LLC-PK, cells but was barely expressed in rat aortic smooth muscle cells and human mesangial cells. By contrast, the ANP-B receptor was the major subtype of the biologically active receptors in rat aortic smooth muscle cells and human mesangial cells. Only a small amount of the ANP-B receptor was detected in PC12 cells, bovine endothelial cells, and LLC-PK, cells. The clearance receptor was abundantly expressed in rat aortic smooth muscle cells and human mesangial cells and was also present in bovine endothelial cells, but it was undetectable in PC12 cells and LLC-PK1 cells. These results demonstrate that the expression of three natriuretic peptide receptors varies from cell to cell, which is relevant to cell- or tissue-specific action of the natriuretic peptide family.

本文言語英語
ページ(範囲)762-765
ページ数4
ジャーナルHypertension
19
6
DOI
出版ステータス出版済み - 6 1992
外部発表はい

All Science Journal Classification (ASJC) codes

  • 内科学

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