Characterization of polypropyleneimine as an alternative transfection reagent

Riku Saeki; Shingo Kobayashi; Rena Shimazui; Teruki Nii; Akihiro Kishimura; Takeshi Mori; Masaru Tanaka; Yoshiki Katayama

doi:10.1007/s44211-023-00284-x

Characterization of polypropyleneimine as an alternative transfection reagent

Riku Saeki, Shingo Kobayashi, Rena Shimazui, Teruki Nii, Akihiro Kishimura, Takeshi Mori, Masaru Tanaka, Yoshiki Katayama

研究成果: ジャーナルへの寄稿 › 学術誌 › 査読

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Polypropyleneimine (PPI) was examined as a transfection reagent comparing with most widely used polymer, polyethyleneimine (PEI). PPI had better responsiveness to the endosomal pH and showed more condensation ability of plasmid DNA than PEI. Although the cytotoxicity of PPI was somewhat higher than PEI, the transfection efficacy of PPI was comparable with PEI or higher than PEI in some cell line. Thus, PPI would be an alternative transfection reagent.

本文言語	英語
ジャーナル	Analytical sciences : the international journal of the Japan Society for Analytical Chemistry
DOI	https://doi.org/10.1007/s44211-023-00284-x
出版ステータス	印刷前の電子出版 - 3月 1 2023

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10.1007/s44211-023-00284-x

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abstract = "Polypropyleneimine (PPI) was examined as a transfection reagent comparing with most widely used polymer, polyethyleneimine (PEI). PPI had better responsiveness to the endosomal pH and showed more condensation ability of plasmid DNA than PEI. Although the cytotoxicity of PPI was somewhat higher than PEI, the transfection efficacy of PPI was comparable with PEI or higher than PEI in some cell line. Thus, PPI would be an alternative transfection reagent.",

author = "Riku Saeki and Shingo Kobayashi and Rena Shimazui and Teruki Nii and Akihiro Kishimura and Takeshi Mori and Masaru Tanaka and Yoshiki Katayama",

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doi = "10.1007/s44211-023-00284-x",

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T1 - Characterization of polypropyleneimine as an alternative transfection reagent

AU - Saeki, Riku

AU - Kobayashi, Shingo

AU - Shimazui, Rena

AU - Nii, Teruki

AU - Kishimura, Akihiro

AU - Mori, Takeshi

AU - Tanaka, Masaru

AU - Katayama, Yoshiki

PY - 2023/3/1

Y1 - 2023/3/1

N2 - Polypropyleneimine (PPI) was examined as a transfection reagent comparing with most widely used polymer, polyethyleneimine (PEI). PPI had better responsiveness to the endosomal pH and showed more condensation ability of plasmid DNA than PEI. Although the cytotoxicity of PPI was somewhat higher than PEI, the transfection efficacy of PPI was comparable with PEI or higher than PEI in some cell line. Thus, PPI would be an alternative transfection reagent.

AB - Polypropyleneimine (PPI) was examined as a transfection reagent comparing with most widely used polymer, polyethyleneimine (PEI). PPI had better responsiveness to the endosomal pH and showed more condensation ability of plasmid DNA than PEI. Although the cytotoxicity of PPI was somewhat higher than PEI, the transfection efficacy of PPI was comparable with PEI or higher than PEI in some cell line. Thus, PPI would be an alternative transfection reagent.

U2 - 10.1007/s44211-023-00284-x

DO - 10.1007/s44211-023-00284-x

M3 - Article

C2 - 36859695

SN - 0910-6340

JO - Analytical Sciences

JF - Analytical Sciences

ER -

Characterization of polypropyleneimine as an alternative transfection reagent

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