Characterization of production of free gluconic acid by Gluconobacter suboxydans adsorbed on ceramic honeycomb monolith

Fumihide Shiraishi, Koei Kawakami, Seiji Kono, Akihiko Tamura, Satoshi Tsuruta, Koichiro Kusunoki

研究成果: Contribution to journalArticle査読

26 被引用数 (Scopus)

抄録

Gluconobacter suboxydans IFO 3290 was immobilized by adsorption on ceramic honeycomb monolith and continuous production of free gluconic acid from glucose was performed in an aerated reactor. The effects of reactor residence time, aeration rate, and glucose concentration were investigated on the gluconic acid yield. Observation of SEM photographs revealed that the cells were adsorbed with a high density not only on the outer surface of the support but also on the inner surface of large pores. From measurement of the number of the adsorbed cells, it was elucidated that the biofilm comprised a monolayer or bilayer of the cells. Maximum specific rate of growth was estimated for the free and adsorbed cells, and the adsorbed cells were found to grow at a fast rate compared with the free cells. In the continuous fermentation performed for one month at the glucose concentration of 100 kg/m3, reactor residence time of 3.5 h and aeration rate of 900 cm3/min, the activity of the adsorbed cells was appreciably stable. The high productivity of 26.3 kg/(m3‐reactor · h) was attained with the gluconic acid yield of 84.6% and glucose conversion of 94%.

本文言語英語
ページ(範囲)1413-1418
ページ数6
ジャーナルBiotechnology and Bioengineering
33
11
DOI
出版ステータス出版済み - 5 1989

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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