Characterization of recombinant amyloidogenic chicken cystatin mutant I66Q expressed in yeast

Jianwei He, Youtao Song, Nobuhiro Ueyama, Akihito Harada, Hiroyuki Azakami, Akio Kato

研究成果: ジャーナルへの寄稿学術誌査読

9 被引用数 (Scopus)

抄録

Amyloidogenic chicken cystatin mutant I66Q (cC I66Q) was successfully secreted by yeasts Pichia pastoris and Saccharomyces cerevisiae. The soluble monomer and dimer forms of amyloidogenic cC I66Q were found in the culture medium, while large amounts of insoluble aggregate and polymeric form cC I66Q besides the monomer and dimer forms were secreted into the culture medium. The amyloidogenic cC I66Q showed a comparable circular dichroism spectrum to that of the wild cystatin, and the monomer form exhibited a similar level of inhibitory activity toward papain, but the dimmer form did not. During storage of amyloidogenic cC I66Q under physiological and acidic conditions, typical binding with Congo red and thioflavin T, and the formation of amyloid fibrils were observed, whereas the characteristic of similar amyloidosis was hardly detected for the wild recombinant cystatin.

本文言語英語
ページ(範囲)477-485
ページ数9
ジャーナルJournal of biochemistry
137
4
DOI
出版ステータス出版済み - 4月 2005
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学

フィンガープリント

「Characterization of recombinant amyloidogenic chicken cystatin mutant I66Q expressed in yeast」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル