Characterization of refolded hen lysozyme variant lacking two outside disulfide bonds

Takatoshi Ohkuri, Taiji Imoto, Tadashi Ueda

研究成果: ジャーナルへの寄稿記事

抜粋

We characterized a refolded hen lysozyme variant containing only two SS-bonds, C64-C80 and C76-C94 (4CAHEL). From CD spectra and its activity, it was found that the refolded 4CAHEL has a structural topology analogous to wild-type lysozyme (WTHEL). Moreover, the refolded 4CAHEL showed no thermal transition, indicating that it had a character like a molten globule.

元の言語英語
ページ(範囲)1206-1208
ページ数3
ジャーナルBioscience, Biotechnology and Biochemistry
69
発行部数6
DOI
出版物ステータス出版済み - 6 1 2005

    フィンガープリント

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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