Characterization of RNA binding protein RBP-P reveals a possible role in rice glutelin gene expression and RNA localization

Kelly A. Doroshenk, Li Tian, Andrew J. Crofts, Toshihiro Kumamaru, Thomas W. Okita

研究成果: ジャーナルへの寄稿記事

12 引用 (Scopus)

抄録

RNA binding proteins (RBPs) play an important role in mRNA metabolism including synthesis, maturation, transport, localization, and stability. In developing rice seeds, RNAs that code for the major storage proteins are transported to specific domains of the cortical endoplasmic reticulum (ER) by a regulated mechanism requiring RNA cis-localization elements, or zipcodes. Putative trans-acting RBPs that recognize prolamine RNA zipcodes required for restricted localization to protein body-ER have previously been identified. Here, we describe the identification of RBP-P using a Northwestern blot approach as an RBP that recognizes and binds to glutelin zipcode RNA, which is required for proper RNA localization to cisternal-ER. RBP-P protein expression coincides with that of glutelin during seed maturation and is localized to both the nucleus and cytosol. RNA-immunoprecipitation and subsequent RT-PCR analysis further demonstrated that RBP-P interacts with glutelin RNAs. In vitro RNA-protein UV-crosslinking assays showed that recombinant RBP-P binds strongly to glutelin mRNA, and in particular, 3′ UTR and zipcode RNA. RBP-P also exhibited strong binding activity to a glutelin intron sequence, suggesting that RBP-P might participate in mRNA splicing. Overall, these results support a multifunctional role for RBP-P in glutelin mRNA metabolism, perhaps in nuclear pre-mRNA splicing and cytosolic localization to the cisternal-ER.

元の言語英語
ページ(範囲)381-394
ページ数14
ジャーナルPlant Molecular Biology
85
発行部数4
DOI
出版物ステータス出版済み - 1 1 2014

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glutelins
RNA-binding proteins
RNA-Binding Proteins
Glutens
Protein Binding
RNA
Gene Expression
rice
gene expression
Endoplasmic Reticulum
endoplasmic reticulum
Messenger RNA
Seeds
Proteins
Oryza
Phenylpropanolamine
protein bodies
metabolism
seed maturation
RNA Precursors

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

これを引用

Characterization of RNA binding protein RBP-P reveals a possible role in rice glutelin gene expression and RNA localization. / Doroshenk, Kelly A.; Tian, Li; Crofts, Andrew J.; Kumamaru, Toshihiro; Okita, Thomas W.

:: Plant Molecular Biology, 巻 85, 番号 4, 01.01.2014, p. 381-394.

研究成果: ジャーナルへの寄稿記事

Doroshenk, Kelly A. ; Tian, Li ; Crofts, Andrew J. ; Kumamaru, Toshihiro ; Okita, Thomas W. / Characterization of RNA binding protein RBP-P reveals a possible role in rice glutelin gene expression and RNA localization. :: Plant Molecular Biology. 2014 ; 巻 85, 番号 4. pp. 381-394.
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