Characterization of side-population cells in human normal endometrium

Kiyoko Kato, Momoko Yoshimoto, Keiji Kato, Sawako Adachi, Asako Yamayoshi, Takahiro Arima, Kazuo Asanoma, Satoru Kyo, Tatsutoshi Nakahata, Norio Wake

研究成果: ジャーナルへの寄稿記事

164 引用 (Scopus)

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Background: It has been proposed that the human endometrium may contain a population of adult stem cells that are responsible for its remarkable regenerative capability. Recently, a subset of stem cells or progenitor cells in adult tissue has been identified as side-population cells (SP cells) displaying low staining with Hoechst 33342 by fluorescence-activated cell sorter (FACS) analysis. In this study, we isolated SP cells from the human endometrium and analysed their properties. Method: Endometrial cells were obtained using enzymatic digestion from uterine hysterectomy for the treatment of uterine myoma and stained with Hoechst 33342 dye either alone or in combination with verapamil. The cells were then analysed using FACS. Results: SP cells were present among normal human endometrial cells. Most SP cells were enriched in the CD9-CD13- fraction. These SP cells showed long-term repopulating properties and produced gland (CD9+)- and stroma (CD13+)-like cells. CD9-CD13- cells isolated from the endometrium also generated gland- or stroma-like cells. Conclusions: SP cells in the human endometrium can function as progenitor cells. This is the first report of the phenotype of SP cells from normal human endometrial cells.

元の言語英語
ページ(範囲)1214-1223
ページ数10
ジャーナルHuman Reproduction
22
発行部数5
DOI
出版物ステータス出版済み - 5 2007

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Obstetrics and Gynaecology

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    Kato, K., Yoshimoto, M., Kato, K., Adachi, S., Yamayoshi, A., Arima, T., Asanoma, K., Kyo, S., Nakahata, T., & Wake, N. (2007). Characterization of side-population cells in human normal endometrium. Human Reproduction, 22(5), 1214-1223. https://doi.org/10.1093/humrep/del514