Characterization of the 5′-flanking region and chromosomal assignment of the human brain natriuretic peptide gene

Y. Ogawa, H. Itoh, O. Nakagawa, G. Shirakami, N. Tamura, T. Yoshimasa, K. Nagata, N. Yoshida, K. Nakao

研究成果: ジャーナルへの寄稿学術誌査読

21 被引用数 (Scopus)

抄録

Brain natriuretic peptide (BNP) is a cardiac hormone that occurs predominantly in the ventricle, and synthesis and secretion of BNP are greatly augmented in patients with congestive heart failure and in animal models of ventricular hypertrophy. In order to elucidate the molecular mechanisms underlying the human BNP gene expression in the heart, the human BNP gene was isolated from a size-selected genomic minilibrary. The 1.9-kb human BNP 5′-flanking region (-1813 to +110) contained an array of putative cis-acting regulatory elements. Various lengths of the cloned 5′-flanking sequences were linked upstream to the bacterial chloramphenicol acetyltransferase (CAT) gene, and their promoter activities were assayed. The 1.9-kb promoter region showed a high-level CAT activity in cultured neonatal rat ventricular cardiocytes. When the CT-rich sequences (-1288 to -1095) were deleted, the high-level activity was reduced to approximately 30%. The 399-bp BNP 5′ flanking region (-289 to +110) showed approximately 10% activity of the 1.9-kb region. Furthermore, using human-rodent somatic hybrid cell lines, the BNP gene was assigned to human chromosome 1, on which the atrial natriuretic peptide gene is localized. The present study leads to a better understanding of the molecular mechanisms for the human BNP gene expression in the heart.

本文言語英語
ページ(範囲)457-463
ページ数7
ジャーナルJournal of Molecular Medicine
73
9
DOI
出版ステータス出版済み - 9月 1 1995
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 分子医療
  • 創薬
  • 遺伝学(臨床)

フィンガープリント

「Characterization of the 5′-flanking region and chromosomal assignment of the human brain natriuretic peptide gene」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル