Characterization of the carotenoid-binding protein of the Y-gene dominant mutants of Bombyx mori

Kozo Tsuchida, Zeina E. Jouni, Jennifer Gardetto, Yutaka Kobayashi, Hiroko Tabunoki, Masaaki Azuma, Hiromu Sugiyama, Naoko Takada, Hideaki Maekawa, Yutaka Banno, Hiroshi Fujii, Hidetoshi Iwano, Michael A. Wells

研究成果: ジャーナルへの寄稿記事

22 引用 (Scopus)

抄録

Carotenoids play important and diverse roles in insects. Recently, we purified and partially characterized a carotenoid-binding protein (CBP) from the wild type of Bombyx mori. In this report, we utilized immunoblotting, ELISA and immunocytochemistry to further characterize and localize the expression of CBP in the larval midgut and silk gland obtained from the wild type and four naturally occurring mutants linked to carotenoids transport. CBP was expressed throughout the 5th stadium, with highest expressions on days 4-5 in the silk gland and days 3-5 in the midgut. Immunoblotting analyses demonstrated the presence of CBP along the middle part of the midgut. Microscopic immunocytochemistry demonstrated that the 33 kDa CBP was uniformly expressed along the brush border of columnar cells in the epithelium of the midgut typifying its function in aiding absorption of dietary carotenoids. Similarly, CBP was highly expressed along the distal membrane of the middle part of the silk gland demonstrating its function in uptake of carotenoids from lipophorin. When the middle silk glands and midguts of the four mutants were incubated with rabbit anti-CBP antibody, only proteins of the Y-gene dominant mutants cross reacted with the antibody further accentuating the hypothesis that the CBP is a Y-gene dependent protein.

元の言語英語
ページ(範囲)363-372
ページ数10
ジャーナルJournal of insect physiology
50
発行部数4
DOI
出版物ステータス出版済み - 4 1 2004

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Dominant Genes
Bombyx
dominant genes
Carotenoids
Bombyx mori
binding proteins
Carrier Proteins
carotenoids
mutants
silk glands
midgut
Silk
immunocytochemistry
immunoblotting
Immunoblotting
Immunohistochemistry
lipophorin
antibodies
Antibodies
Microvilli

All Science Journal Classification (ASJC) codes

  • Physiology
  • Insect Science

これを引用

Tsuchida, K., Jouni, Z. E., Gardetto, J., Kobayashi, Y., Tabunoki, H., Azuma, M., ... Wells, M. A. (2004). Characterization of the carotenoid-binding protein of the Y-gene dominant mutants of Bombyx mori. Journal of insect physiology, 50(4), 363-372. https://doi.org/10.1016/j.jinsphys.2004.02.006

Characterization of the carotenoid-binding protein of the Y-gene dominant mutants of Bombyx mori. / Tsuchida, Kozo; Jouni, Zeina E.; Gardetto, Jennifer; Kobayashi, Yutaka; Tabunoki, Hiroko; Azuma, Masaaki; Sugiyama, Hiromu; Takada, Naoko; Maekawa, Hideaki; Banno, Yutaka; Fujii, Hiroshi; Iwano, Hidetoshi; Wells, Michael A.

:: Journal of insect physiology, 巻 50, 番号 4, 01.04.2004, p. 363-372.

研究成果: ジャーナルへの寄稿記事

Tsuchida, K, Jouni, ZE, Gardetto, J, Kobayashi, Y, Tabunoki, H, Azuma, M, Sugiyama, H, Takada, N, Maekawa, H, Banno, Y, Fujii, H, Iwano, H & Wells, MA 2004, 'Characterization of the carotenoid-binding protein of the Y-gene dominant mutants of Bombyx mori', Journal of insect physiology, 巻. 50, 番号 4, pp. 363-372. https://doi.org/10.1016/j.jinsphys.2004.02.006
Tsuchida, Kozo ; Jouni, Zeina E. ; Gardetto, Jennifer ; Kobayashi, Yutaka ; Tabunoki, Hiroko ; Azuma, Masaaki ; Sugiyama, Hiromu ; Takada, Naoko ; Maekawa, Hideaki ; Banno, Yutaka ; Fujii, Hiroshi ; Iwano, Hidetoshi ; Wells, Michael A. / Characterization of the carotenoid-binding protein of the Y-gene dominant mutants of Bombyx mori. :: Journal of insect physiology. 2004 ; 巻 50, 番号 4. pp. 363-372.
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abstract = "Carotenoids play important and diverse roles in insects. Recently, we purified and partially characterized a carotenoid-binding protein (CBP) from the wild type of Bombyx mori. In this report, we utilized immunoblotting, ELISA and immunocytochemistry to further characterize and localize the expression of CBP in the larval midgut and silk gland obtained from the wild type and four naturally occurring mutants linked to carotenoids transport. CBP was expressed throughout the 5th stadium, with highest expressions on days 4-5 in the silk gland and days 3-5 in the midgut. Immunoblotting analyses demonstrated the presence of CBP along the middle part of the midgut. Microscopic immunocytochemistry demonstrated that the 33 kDa CBP was uniformly expressed along the brush border of columnar cells in the epithelium of the midgut typifying its function in aiding absorption of dietary carotenoids. Similarly, CBP was highly expressed along the distal membrane of the middle part of the silk gland demonstrating its function in uptake of carotenoids from lipophorin. When the middle silk glands and midguts of the four mutants were incubated with rabbit anti-CBP antibody, only proteins of the Y-gene dominant mutants cross reacted with the antibody further accentuating the hypothesis that the CBP is a Y-gene dependent protein.",
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AU - Kobayashi, Yutaka

AU - Tabunoki, Hiroko

AU - Azuma, Masaaki

AU - Sugiyama, Hiromu

AU - Takada, Naoko

AU - Maekawa, Hideaki

AU - Banno, Yutaka

AU - Fujii, Hiroshi

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