TY - JOUR
T1 - Characterization of the genomic structure and expression of the mouse Apex2 gene
AU - Ide, Yasuhito
AU - Tsuchimoto, Daisuke
AU - Tominaga, Yohei
AU - Iwamoto, Yukihide
AU - Nakabeppu, Yusaku
N1 - Funding Information:
We extend our special thanks to Masato Furuichi, Kunihiko Sakumi, and Yoshinori Ohnishi for helpful discussions; Toshiki Tsurimoto for recombinant human PCNA preparation; Daniel Nathans for COS-1 and BALB/c 3T3 cells; Motoya Katsuki for the CCE ES cells; Kenji Nakamura and Norihiko Kinoshita for assistance in ES cell culture; Masafumi Sasaki for assistance with the electron microscopy; and Brian Quinn for useful comments on the manuscript. This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan and the Japan Society for the Promotion of Science.
PY - 2003/1/1
Y1 - 2003/1/1
N2 - We isolated a mouse cDNA encoding APEX2 protein and demonstrated that APEX2 binds to PCNA. The level of Apex2 mRNA was high in the thymus, bone marrow, spleen, and kidney in adult mice. Apex2 consists of six exons and is flanked on the 3′ end by Alas2 on X chromosome 63.0. Furthermore, Apex2 is flanked on the 5′ end by a novel gene with a 106-bp intergenic sequence. We disrupted Apex2 in embryonic stem cells derived from a male mouse, and a 55-kDa APEX2 protein was detected in the nuclei of Apex2+ but not Apex2-disrupted cells. Immunoelectron microscopy revealed that APEX2 is also localized in the mitochondria of Apex2+ cells. In serum-stimulated BALB/c 3T3 cells, the level of Apex2 mRNA was transiently increased and the level of APEX2 reached a maximum in the late S phase, thus indicating that APEX2 may participate in postreplicative base excision repair.
AB - We isolated a mouse cDNA encoding APEX2 protein and demonstrated that APEX2 binds to PCNA. The level of Apex2 mRNA was high in the thymus, bone marrow, spleen, and kidney in adult mice. Apex2 consists of six exons and is flanked on the 3′ end by Alas2 on X chromosome 63.0. Furthermore, Apex2 is flanked on the 5′ end by a novel gene with a 106-bp intergenic sequence. We disrupted Apex2 in embryonic stem cells derived from a male mouse, and a 55-kDa APEX2 protein was detected in the nuclei of Apex2+ but not Apex2-disrupted cells. Immunoelectron microscopy revealed that APEX2 is also localized in the mitochondria of Apex2+ cells. In serum-stimulated BALB/c 3T3 cells, the level of Apex2 mRNA was transiently increased and the level of APEX2 reached a maximum in the late S phase, thus indicating that APEX2 may participate in postreplicative base excision repair.
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U2 - 10.1016/S0888-7543(02)00009-5
DO - 10.1016/S0888-7543(02)00009-5
M3 - Article
C2 - 12573260
AN - SCOPUS:0037289280
SN - 0888-7543
VL - 81
SP - 47
EP - 57
JO - Genomics
JF - Genomics
IS - 1
ER -