Characterization of the mouse gene for the U-box-type ubiquitin ligase UFD2a

Chie Kaneko, Shigetsugu Hatakeyama, Masaki Matsumoto, Masayoshi Yada, Keiko Nakayama, Keiichi Nakayama

研究成果: ジャーナルへの寄稿記事

47 引用 (Scopus)

抄録

UFD2a is a mammalian homolog of Saccharomyces cerevisiae Ufd2, originally described as an E4 ubiquitination factor. UFD2a belongs to the U-box family of ubiquitin ligases (E3s) and likely functions as both an E3 and E4. We have isolated and characterized the mouse gene (Ube4b) for UFD2a. A full-length (∼5700bp) Ube4b cDNA was isolated and the corresponding gene spans 100kb, comprising 27 exons. Luciferase reporter gene analysis of the 5 flanking region of Ube4b revealed that nucleotides -1018 to -943 (relative to the translation initiation site) possess promoter activity. This functional sequence contains two putative Sp1 binding sites but not a TATA box. Immunoblot and immunohistochemical analyses revealed that UFD2a is expressed predominantly in the neuronal tissues. We also show that UFD2a interacts with VCP (a AAA-family ATPase) that is thought to mediate protein folding. These data implicate UFD2a in the degradation of neuronal proteins by the ubiquitin-proteasome pathway.

元の言語英語
ページ(範囲)297-304
ページ数8
ジャーナルBiochemical and Biophysical Research Communications
300
発行部数2
DOI
出版物ステータス出版済み - 1 10 2003

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Ligases
Ubiquitin
Genes
TATA Box
5' Flanking Region
Ubiquitination
Protein Folding
Proteasome Endopeptidase Complex
Luciferases
Reporter Genes
Proteolysis
Saccharomyces cerevisiae
Adenosine Triphosphatases
Protein folding
Exons
Nucleotides
Complementary DNA
Binding Sites
Yeast
Tissue

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

Characterization of the mouse gene for the U-box-type ubiquitin ligase UFD2a. / Kaneko, Chie; Hatakeyama, Shigetsugu; Matsumoto, Masaki; Yada, Masayoshi; Nakayama, Keiko; Nakayama, Keiichi.

:: Biochemical and Biophysical Research Communications, 巻 300, 番号 2, 10.01.2003, p. 297-304.

研究成果: ジャーナルへの寄稿記事

Kaneko, Chie ; Hatakeyama, Shigetsugu ; Matsumoto, Masaki ; Yada, Masayoshi ; Nakayama, Keiko ; Nakayama, Keiichi. / Characterization of the mouse gene for the U-box-type ubiquitin ligase UFD2a. :: Biochemical and Biophysical Research Communications. 2003 ; 巻 300, 番号 2. pp. 297-304.
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