Characterization of the product of the gtfS gene of Streptococcus downei, a primer-independent enzyme synthesizing oligo-isomaltosaccharides

R. R.B. Russell, M. L. Gilpin, N. Hanada, Y. Yamashita, Y. Shibata, T. Takehara

研究成果: ジャーナルへの寄稿記事

9 引用 (Scopus)

抄録

The gtfS gene, coding for a glucosyltransferase which synthesizes water-soluble glucan and previously cloned from Streptococcus downei strain MFe28 (mutans serotype h) into a bacteriophage vector, was subcloned into a plasmid vector. The gtfS gene products expressed in Escherichia coli were compared to the primer-independent, oligo-isomaltosaccharide synthase in Streptococcus sobrinus strain AHT (mutans serotype g) and shown to resemble it closely in molecular mass, isoelectric point, immunological properties, optimum pH and K(m) values. The glucans produced from sucrose by the gtfS gene products are α-1,6-linked linear oligo-isomaltosaccharides without any branching sites. A similar gtfS gene was also detected on chromosomal DNA from S. sobrinus strain AHT.

元の言語英語
ページ(範囲)1631-1637
ページ数7
ジャーナルJournal of General Microbiology
136
発行部数8
DOI
出版物ステータス出版済み - 1 1 1990

Fingerprint

Streptococcus
Streptococcus sobrinus
Glucans
Enzymes
Genes
Glucosyltransferases
Isoelectric Point
Bacteriophages
Sucrose
Plasmids
Escherichia coli
Water
DNA
Serogroup

All Science Journal Classification (ASJC) codes

  • Microbiology

これを引用

Characterization of the product of the gtfS gene of Streptococcus downei, a primer-independent enzyme synthesizing oligo-isomaltosaccharides. / Russell, R. R.B.; Gilpin, M. L.; Hanada, N.; Yamashita, Y.; Shibata, Y.; Takehara, T.

:: Journal of General Microbiology, 巻 136, 番号 8, 01.01.1990, p. 1631-1637.

研究成果: ジャーナルへの寄稿記事

@article{31c40fe165764de78888ef12fbed1e93,
title = "Characterization of the product of the gtfS gene of Streptococcus downei, a primer-independent enzyme synthesizing oligo-isomaltosaccharides",
abstract = "The gtfS gene, coding for a glucosyltransferase which synthesizes water-soluble glucan and previously cloned from Streptococcus downei strain MFe28 (mutans serotype h) into a bacteriophage vector, was subcloned into a plasmid vector. The gtfS gene products expressed in Escherichia coli were compared to the primer-independent, oligo-isomaltosaccharide synthase in Streptococcus sobrinus strain AHT (mutans serotype g) and shown to resemble it closely in molecular mass, isoelectric point, immunological properties, optimum pH and K(m) values. The glucans produced from sucrose by the gtfS gene products are α-1,6-linked linear oligo-isomaltosaccharides without any branching sites. A similar gtfS gene was also detected on chromosomal DNA from S. sobrinus strain AHT.",
author = "Russell, {R. R.B.} and Gilpin, {M. L.} and N. Hanada and Y. Yamashita and Y. Shibata and T. Takehara",
year = "1990",
month = "1",
day = "1",
doi = "10.1099/00221287-136-8-1631",
language = "English",
volume = "136",
pages = "1631--1637",
journal = "Microbiology",
issn = "1350-0872",
publisher = "Society for General Microbiology",
number = "8",

}

TY - JOUR

T1 - Characterization of the product of the gtfS gene of Streptococcus downei, a primer-independent enzyme synthesizing oligo-isomaltosaccharides

AU - Russell, R. R.B.

AU - Gilpin, M. L.

AU - Hanada, N.

AU - Yamashita, Y.

AU - Shibata, Y.

AU - Takehara, T.

PY - 1990/1/1

Y1 - 1990/1/1

N2 - The gtfS gene, coding for a glucosyltransferase which synthesizes water-soluble glucan and previously cloned from Streptococcus downei strain MFe28 (mutans serotype h) into a bacteriophage vector, was subcloned into a plasmid vector. The gtfS gene products expressed in Escherichia coli were compared to the primer-independent, oligo-isomaltosaccharide synthase in Streptococcus sobrinus strain AHT (mutans serotype g) and shown to resemble it closely in molecular mass, isoelectric point, immunological properties, optimum pH and K(m) values. The glucans produced from sucrose by the gtfS gene products are α-1,6-linked linear oligo-isomaltosaccharides without any branching sites. A similar gtfS gene was also detected on chromosomal DNA from S. sobrinus strain AHT.

AB - The gtfS gene, coding for a glucosyltransferase which synthesizes water-soluble glucan and previously cloned from Streptococcus downei strain MFe28 (mutans serotype h) into a bacteriophage vector, was subcloned into a plasmid vector. The gtfS gene products expressed in Escherichia coli were compared to the primer-independent, oligo-isomaltosaccharide synthase in Streptococcus sobrinus strain AHT (mutans serotype g) and shown to resemble it closely in molecular mass, isoelectric point, immunological properties, optimum pH and K(m) values. The glucans produced from sucrose by the gtfS gene products are α-1,6-linked linear oligo-isomaltosaccharides without any branching sites. A similar gtfS gene was also detected on chromosomal DNA from S. sobrinus strain AHT.

UR - http://www.scopus.com/inward/record.url?scp=0025046269&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025046269&partnerID=8YFLogxK

U2 - 10.1099/00221287-136-8-1631

DO - 10.1099/00221287-136-8-1631

M3 - Article

C2 - 2148181

AN - SCOPUS:0025046269

VL - 136

SP - 1631

EP - 1637

JO - Microbiology

JF - Microbiology

SN - 1350-0872

IS - 8

ER -