Characterization of the RAGE-binding protein, Strongyloides venestatin, produced by the silkworm-baculovirus expression system

Daigo Tsubokawa, Jae Man Lee, Takeshi Hatta, Fusako Mikami, Haruhiko Maruyama, Takeshi Arakawa, Takahiro Kusakabe, Naotoshi Tsuji

研究成果: Contribution to journalArticle査読

2 被引用数 (Scopus)

抄録

The receptor for advanced glycation end products (RAGE) recognizes Ca++-binding proteins, such as members of the S100 protein family released by dead or devitalized tissues, and plays an important role in inflammatory responses. We recently identified the Ca++-binding protein, venestatin, secreted from the rodent parasitic nematode, Strongyloides venezuelensis. We herein characterized recombinant venestatin, which is abundantly produced by the silkworm-baculovirus expression system (silkworm-BES), particularly in its interaction with RAGE. Venestatin from silkworm-BES possessed a binding capacity with Ca++ ions and vaccine immunogenicity against S. venezuelensis larvae in mice, which is similar to venestatin produced by the E. coli expression system (EES). Venestatin from silkworm-BES had a higher affinity for human recombinant RAGE than that from EES, and their affinities were Ca++-dependent. RAGE in the mouse lung co-immunoprecipitated with venestatin from silkworm-BES administered intranasally, indicating that it bound endogenous mouse RAGE. The present results suggest that venestatin from silkworm-BES affects RAGE-mediated pathological processes.

本文言語英語
論文番号103964
ジャーナルInfection, Genetics and Evolution
75
DOI
出版ステータス出版済み - 11 2019

All Science Journal Classification (ASJC) codes

  • 微生物学
  • 生態、進化、行動および分類学
  • 分子生物学
  • 遺伝学
  • 微生物学(医療)
  • 感染症

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