Characterization of the RAGE-binding protein, Strongyloides venestatin, produced by the silkworm-baculovirus expression system

Daigo Tsubokawa, Man Lee, Takeshi Hatta, Fusako Mikami, Haruhiko Maruyama, Takeshi Arakawa, Takahiro Kusakabe, Naotoshi Tsuji

研究成果: ジャーナルへの寄稿記事

抄録

The receptor for advanced glycation end products (RAGE) recognizes Ca++-binding proteins, such as members of the S100 protein family released by dead or devitalized tissues, and plays an important role in inflammatory responses. We recently identified the Ca++-binding protein, venestatin, secreted from the rodent parasitic nematode, Strongyloides venezuelensis. We herein characterized recombinant venestatin, which is abundantly produced by the silkworm-baculovirus expression system (silkworm-BES), particularly in its interaction with RAGE. Venestatin from silkworm-BES possessed a binding capacity with Ca++ ions and vaccine immunogenicity against S. venezuelensis larvae in mice, which is similar to venestatin produced by the E. coli expression system (EES). Venestatin from silkworm-BES had a higher affinity for human recombinant RAGE than that from EES, and their affinities were Ca++-dependent. RAGE in the mouse lung co-immunoprecipitated with venestatin from silkworm-BES administered intranasally, indicating that it bound endogenous mouse RAGE. The present results suggest that venestatin from silkworm-BES affects RAGE-mediated pathological processes.

元の言語英語
記事番号103964
ジャーナルInfection, Genetics and Evolution
75
DOI
出版物ステータス出版済み - 11 1 2019

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Strongyloides
Bombyx
Baculoviridae
silkworms
binding proteins
Carrier Proteins
receptors
protein
mice
S100 Proteins
animal parasitic nematodes
Pathologic Processes
vaccine
binding capacity
Larva
Rodentia
rodent
nematode
advanced glycation end products
Advanced Glycosylation End Product-Specific Receptor

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Microbiology (medical)
  • Infectious Diseases

これを引用

Characterization of the RAGE-binding protein, Strongyloides venestatin, produced by the silkworm-baculovirus expression system. / Tsubokawa, Daigo; Lee, Man; Hatta, Takeshi; Mikami, Fusako; Maruyama, Haruhiko; Arakawa, Takeshi; Kusakabe, Takahiro; Tsuji, Naotoshi.

:: Infection, Genetics and Evolution, 巻 75, 103964, 01.11.2019.

研究成果: ジャーナルへの寄稿記事

Tsubokawa, Daigo ; Lee, Man ; Hatta, Takeshi ; Mikami, Fusako ; Maruyama, Haruhiko ; Arakawa, Takeshi ; Kusakabe, Takahiro ; Tsuji, Naotoshi. / Characterization of the RAGE-binding protein, Strongyloides venestatin, produced by the silkworm-baculovirus expression system. :: Infection, Genetics and Evolution. 2019 ; 巻 75.
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