Characterization of the roles of DNA polymerases, clamp, and clamp loaders during s-phase progression and cell cycle regulation in the silkworm, bombyx mori

Masato Hino, Daisuke Morokuma, Hiroaki Mon, Man Lee, Takahiro Kusakabe

研究成果: ジャーナルへの寄稿記事

抄録

DNA replication is one of key event in cell-cycle progression, yet due to their importance and lethality, the chronological phenotypes of DNA synthesis machineries after the depletion of corresponding genes have proved difficult to study. In the present study, mRNAs for three DNA polymerases, a clamp, and three clamp loaders were gradually depleted from cultured silkworm cells by soaking RNAi. Interestingly, the depletion of these DNA synthesis factors had different effects on the cell growth rate and arrest of cell-cycle progression during time-lapse observation. The depletion of DNA polymerases immediately arrested the cell-cycle progression at the S phase, while that of PCNA, a DNA clamp, required more time to slow cell growth and finally induced apoptosis. Surprisingly, silkworm cells continued to undergo several rounds of cell division when the components of clamp loaders were knocked down.

元の言語英語
ページ(範囲)21-29
ページ数9
ジャーナルJournal of Insect Biotechnology and Sericology
85
発行部数2
DOI
出版物ステータス出版済み - 1 1 2016

Fingerprint

loaders
Loaders
Bombyx
DNA-directed DNA polymerase
Clamping devices
DNA-Directed DNA Polymerase
Bombyx mori
silkworms
cell cycle
Cell Cycle
DNA
Cells
cell growth
synthesis
Fibroblast Growth Factors
Proliferating Cell Nuclear Antigen
Cell growth
DNA replication
Growth
RNA Interference

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Business, Management and Accounting(all)
  • Agricultural and Biological Sciences(all)
  • Insect Science
  • Industrial and Manufacturing Engineering

これを引用

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abstract = "DNA replication is one of key event in cell-cycle progression, yet due to their importance and lethality, the chronological phenotypes of DNA synthesis machineries after the depletion of corresponding genes have proved difficult to study. In the present study, mRNAs for three DNA polymerases, a clamp, and three clamp loaders were gradually depleted from cultured silkworm cells by soaking RNAi. Interestingly, the depletion of these DNA synthesis factors had different effects on the cell growth rate and arrest of cell-cycle progression during time-lapse observation. The depletion of DNA polymerases immediately arrested the cell-cycle progression at the S phase, while that of PCNA, a DNA clamp, required more time to slow cell growth and finally induced apoptosis. Surprisingly, silkworm cells continued to undergo several rounds of cell division when the components of clamp loaders were knocked down.",
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AU - Mon, Hiroaki

AU - Lee, Man

AU - Kusakabe, Takahiro

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