抄録
DNA replication is one of key event in cell-cycle progression, yet due to their importance and lethality, the chronological phenotypes of DNA synthesis machineries after the depletion of corresponding genes have proved difficult to study. In the present study, mRNAs for three DNA polymerases, a clamp, and three clamp loaders were gradually depleted from cultured silkworm cells by soaking RNAi. Interestingly, the depletion of these DNA synthesis factors had different effects on the cell growth rate and arrest of cell-cycle progression during time-lapse observation. The depletion of DNA polymerases immediately arrested the cell-cycle progression at the S phase, while that of PCNA, a DNA clamp, required more time to slow cell growth and finally induced apoptosis. Surprisingly, silkworm cells continued to undergo several rounds of cell division when the components of clamp loaders were knocked down.
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 21-29 |
| ページ数 | 9 |
| ジャーナル | Journal of Insect Biotechnology and Sericology |
| 巻 | 85 |
| 発行部数 | 2 |
| DOI | |
| 出版物ステータス | 出版済み - 1 1 2016 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Applied Microbiology and Biotechnology
- Business, Management and Accounting(all)
- Agricultural and Biological Sciences(all)
- Insect Science
- Industrial and Manufacturing Engineering
これを引用
Characterization of the roles of DNA polymerases, clamp, and clamp loaders during s-phase progression and cell cycle regulation in the silkworm, bombyx mori. / Hino, Masato; Morokuma, Daisuke; Mon, Hiroaki; Lee, Man; Kusakabe, Takahiro.
：: Journal of Insect Biotechnology and Sericology, 巻 85, 番号 2, 01.01.2016, p. 21-29.研究成果: ジャーナルへの寄稿 › 記事
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TY - JOUR
T1 - Characterization of the roles of DNA polymerases, clamp, and clamp loaders during s-phase progression and cell cycle regulation in the silkworm, bombyx mori
AU - Hino, Masato
AU - Morokuma, Daisuke
AU - Mon, Hiroaki
AU - Lee, Man
AU - Kusakabe, Takahiro
PY - 2016/1/1
Y1 - 2016/1/1
N2 - DNA replication is one of key event in cell-cycle progression, yet due to their importance and lethality, the chronological phenotypes of DNA synthesis machineries after the depletion of corresponding genes have proved difficult to study. In the present study, mRNAs for three DNA polymerases, a clamp, and three clamp loaders were gradually depleted from cultured silkworm cells by soaking RNAi. Interestingly, the depletion of these DNA synthesis factors had different effects on the cell growth rate and arrest of cell-cycle progression during time-lapse observation. The depletion of DNA polymerases immediately arrested the cell-cycle progression at the S phase, while that of PCNA, a DNA clamp, required more time to slow cell growth and finally induced apoptosis. Surprisingly, silkworm cells continued to undergo several rounds of cell division when the components of clamp loaders were knocked down.
AB - DNA replication is one of key event in cell-cycle progression, yet due to their importance and lethality, the chronological phenotypes of DNA synthesis machineries after the depletion of corresponding genes have proved difficult to study. In the present study, mRNAs for three DNA polymerases, a clamp, and three clamp loaders were gradually depleted from cultured silkworm cells by soaking RNAi. Interestingly, the depletion of these DNA synthesis factors had different effects on the cell growth rate and arrest of cell-cycle progression during time-lapse observation. The depletion of DNA polymerases immediately arrested the cell-cycle progression at the S phase, while that of PCNA, a DNA clamp, required more time to slow cell growth and finally induced apoptosis. Surprisingly, silkworm cells continued to undergo several rounds of cell division when the components of clamp loaders were knocked down.
UR - http://www.scopus.com/inward/record.url?scp=84991677520&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84991677520&partnerID=8YFLogxK
U2 - 10.11416/jibs.85.2_021
DO - 10.11416/jibs.85.2_021
M3 - Article
AN - SCOPUS:84991677520
VL - 85
SP - 21
EP - 29
JO - Journal of Insect Biotechnology and Sericology
JF - Journal of Insect Biotechnology and Sericology
SN - 1346-8073
IS - 2
ER -