Characterization of transgenic tobacco with an increased α-linolenic acid level

Tatsurou Hamada, Hiroaki Kodama, Keizo Takeshita, Hideo Utsumi, Koh Iba

研究成果: ジャーナルへの寄稿学術誌査読

50 被引用数 (Scopus)

抄録

Microsomal ω-3 fatty acid desaturase catalyzes the conversion of 18:2 (linoleic acid) to 18:3 (α-linolenic acid) in phospholipids, which are the main constituents of extrachloroplast membranes. Transgenic tobacco (Nicotiana tabacum) plants with increased 18:3 contents (designated SIIn plants) were produced through the introduction of a construct with the tobacco microsomal ω-3 fatty acid desaturase gene under the control of the highly efficient promoter containing the E12Ω sequence. 18:3 contents in the SIIn plants were increased by about 40% in roots and by about 10% in leaves compared with the control plants. With regard to growth at 15°C and 25°C and the ability to tolerate chilling at 1°C and 5°C, there were no discernible differences between the SIIn and the control plants. Freezing tolerance in leaves and roots, which was assessed by electrolyte leakage, was almost the same between the SIIn and the control plants. The fluidity of plasma membrane from the SIIn plants was almost the same as that of the control plants. These results indicate that an increase in the 18:3 level in phospholipids is not directly involved in compensation for the diminishment in growth or membrane properties observed under low temperatures.

本文言語英語
ページ(範囲)591-598
ページ数8
ジャーナルPlant physiology
118
2
DOI
出版ステータス出版済み - 1998

!!!All Science Journal Classification (ASJC) codes

  • 生理学
  • 遺伝学
  • 植物科学

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