Characterization of two rice GH18 chitinases belonging to family 8 of plant pathogenesis-related proteins

Jun Tanaka, Tomoya Takashima, Naojiro Abe, Tamo Fukamizo, Tomoyuki Numata, Takayuki Ohnuma

研究成果: ジャーナルへの寄稿学術誌査読

抄録

Two rice GH18 chitinases, Oschib1 and Oschib2, belonging to family 8 of plant pathogenesis-related proteins (PR proteins) were expressed, purified, and characterized. These enzymes, which have the structural features of class IIIb chitinases, preferentially cleaved the second glycosidic linkage from the non-reducing end of substrate chitin oligosaccharides as opposed to rice class IIIa enzymes, OsChib3a and OsChib3b, which mainly cleaved the fourth linkage from the non-reducing end of chitin hexasaccharide [(GlcNAc)6]. Oschib1 and Oschiab2 inhibited the growth of Fusarium solani, but showed only a weak or no antifungal activity against Aspergillus niger and Trichoderma viride on the agar plates. Structural analysis of Oschib1 and Oschib2 revealed that these enzymes have two large loops extruded from the (β/α)8 TIM-barrel fold, which are absent in the structures of class IIIa chitinases. The differences in the cleavage site preferences toward chitin oligosaccharides between plant class IIIa and IIIb chitinases are likely attributed to the additional loop structures found in the IIIb enzymes. The class IIIb chitinases, Oschib1 and Oschib2, seem to play important roles for the effective hydrolysis of chitin oligosaccharides released from the cell wall of the pathogenic fungi by the cooperative actions with the extracellular chitinases in rice.

本文言語英語
論文番号111524
ジャーナルPlant Science
326
DOI
出版ステータス出版済み - 1月 2023

!!!All Science Journal Classification (ASJC) codes

  • 遺伝学
  • 農業および作物学
  • 植物科学

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