Chemiluminescence immunoassay for a nonionic surfactant using a compact disc-type microfluidic platform

Shuai Guo, Koji Nakano, Hizuru Nakajima, Katsumi Uchiyama, Akihide Hemmi, Yoshikazu Yamasaki, Shigeharu Morooka, Ryoichi Ishimatsu, Toshihiko Imato

    研究成果: ジャーナルへの寄稿学術誌査読

    3 被引用数 (Scopus)

    抄録

    A simple and pump-free chemiluminescence immunoassay based on a compact disc (CD)-type microfluidic platform for the determination of alkylphenol polyethoxylates (APnEOs) is described. The method is based on a competitive immunoreaction of the anti- APnEOs antibody immobilized on the magnetic microbeads between APnEOs and horseradish peroxidase (HRP)-labeled APnEOs in the sample solution. The luminol solution containing H 2O 2 and enhancer is caused to flow from one reservoir in the platform to another by appropriate adjustment of the speed of the rotation of the disc. The detection limit was similar to that reported in our previous paper, i.e., 10 ppb according to IC 80.

    本文言語英語
    ページ(範囲)2027-2043
    ページ数17
    ジャーナルPure and Applied Chemistry
    84
    10
    DOI
    出版ステータス出版済み - 2012

    !!!All Science Journal Classification (ASJC) codes

    • 化学 (全般)
    • 化学工学(全般)

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