Chemistry of the bleomycin-induced alkali-labile DNA lesion

Mariko Aso, Mitsumasa Kondo, Hiroshi Suemune, Sidney M. Hecht

研究成果: ジャーナルへの寄稿学術誌査読

39 被引用数 (Scopus)

抄録

Treatment of B-form DNA with the antitumor antibiotic bleomycin in the presence of Fe2+ and O2 affords both DNA strand scission and the formation of alkali-labile lesions, the proportion of which is quite sensitive to the concentration of O2 present. The alkali-labile lesions can undergo fragmentation cleanly in the presence of n-butylamine to afford DNA fragments containing 5'- and 3'-phosphate termini at the site of the alkali-labile lesion. The mechanism of decomposition of the alkali-labile lesion was studied, leading to identification of a putative intermediate that is converted readily to an (oligo)nucleotide 3'-phosphate in the presence of n- BuNH2, as well as the identification of the byproduct of the fragmentation reaction containing the carbon atoms originally present within the alkali- labile lesion.

本文言語英語
ページ(範囲)9023-9033
ページ数11
ジャーナルJournal of the American Chemical Society
121
39
DOI
出版ステータス出版済み - 10月 6 1999

!!!All Science Journal Classification (ASJC) codes

  • 触媒
  • 化学 (全般)
  • 生化学
  • コロイド化学および表面化学

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