Chemistry of the bleomycin-induced alkali-labile DNA lesion

Mariko Aso, Mitsumasa Kondo, Hiroshi Suemune, Sidney M. Hecht

研究成果: ジャーナルへの寄稿記事

35 引用 (Scopus)

抄録

Treatment of B-form DNA with the antitumor antibiotic bleomycin in the presence of Fe2+ and O2 affords both DNA strand scission and the formation of alkali-labile lesions, the proportion of which is quite sensitive to the concentration of O2 present. The alkali-labile lesions can undergo fragmentation cleanly in the presence of n-butylamine to afford DNA fragments containing 5'- and 3'-phosphate termini at the site of the alkali-labile lesion. The mechanism of decomposition of the alkali-labile lesion was studied, leading to identification of a putative intermediate that is converted readily to an (oligo)nucleotide 3'-phosphate in the presence of n- BuNH2, as well as the identification of the byproduct of the fragmentation reaction containing the carbon atoms originally present within the alkali- labile lesion.

元の言語英語
ページ(範囲)9023-9033
ページ数11
ジャーナルJournal of the American Chemical Society
121
発行部数39
DOI
出版物ステータス出版済み - 10 6 1999

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Bleomycin
Alkalies
DNA
Phosphates
Antibiotics
Nucleotides
Byproducts
B-Form DNA
Decomposition
Atoms
Carbon
Anti-Bacterial Agents

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

これを引用

Chemistry of the bleomycin-induced alkali-labile DNA lesion. / Aso, Mariko; Kondo, Mitsumasa; Suemune, Hiroshi; Hecht, Sidney M.

:: Journal of the American Chemical Society, 巻 121, 番号 39, 06.10.1999, p. 9023-9033.

研究成果: ジャーナルへの寄稿記事

Aso, Mariko ; Kondo, Mitsumasa ; Suemune, Hiroshi ; Hecht, Sidney M. / Chemistry of the bleomycin-induced alkali-labile DNA lesion. :: Journal of the American Chemical Society. 1999 ; 巻 121, 番号 39. pp. 9023-9033.
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