Chemoenzymatic synthesis of neoglycoproteins using transglycosylation with endo-β-N-acetylglucosaminidase A

Kiyotaka Fujita, Kaoru Takegawa

研究成果: ジャーナルへの寄稿学術誌査読

17 被引用数 (Scopus)

抄録

A novel chemoenzymatic approach to synthesize neoglycoproteins containing high-mannose-type oligosaccharides is described. p-Isothiocyanatophenyl-β-D-glucopyranoside (Glc-ITC) was transferred to the reducing end of the high-mannose-type oligosaccharides using a transglycosylation activity of endo-β-N-acetylglucosaminidase A (Endo-A). A novel oligosaccharide, Man6GlcNAc-Glc-ITC, was synthesized as a coupling reagent for lysyl and N-terminal residues of the protein moiety. The neoglycoconjugate was coupled with several nonglycosylated proteins such as ribonuclease A, lysozyme, and α-lactalbumin. Between one and four high-mannose-type oligosaccharides were incorporated per molecule of these proteins. This method should be very useful for the synthesis of neoglycoproteins with homogeneous high-mannose-type oligosaccharides.

本文言語英語
ページ(範囲)678-682
ページ数5
ジャーナルBiochemical and Biophysical Research Communications
282
3
DOI
出版ステータス出版済み - 2001
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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