Chicken oviduct-specific expression of transgene by a hybrid ovalbumin enhancer and the Tet expression system

Daisuke Kodama, Daisuke Nishimiya, Ken ichi Nishijima, Yuuki Okino, Yujin Inayoshi, Yasuhiro Kojima, Ken ichiro Ono, Makoto Motono, Katsuhide Miyake, Yoshinori Kawabe, Kenji Kyogoku, Takashi Yamashita, Masamichi Kamihira, Shinji Iijima

研究成果: ジャーナルへの寄稿記事

12 引用 (Scopus)

抄録

We generated genetically manipulated chickens and quail by infecting them with a retroviral vector expressing the human growth hormone under the control of chicken ovalbumin promoter/enhancer up to - 3861. bp from the transcriptional start site. The growth hormone was expressed in an oviduct-specific manner and was found in egg white, although its level was low. The DNA sequence of the integrated form of the viral vector in the packaging cells was shown to be truncated and contained only the sequence spanning - 3861 to - 1569. bp. This represented only the DNase I hypersensitive site (DHS) III of the 4 DHSs and lacked the proximal promoter of the ovalbumin control region. We found several TATA-like and other promoter motifs of approximately - 1800. bp and considered that these promoter motifs and DHS III may cause weak but oviduct-specific expression of the growth hormone. To prove this hypothesis and apply this system to oviduct-specific expression of the transgene, the truncated regulatory sequence was fused to an artificial transactivator-promoter system. In this system, initial weak but oviduct-specific expression of the Tet activator from the promoter element in the ovalbumin control sequence triggered a self-amplifying cycle of expression. DsRed was specifically expressed in oviduct cells of genetically manipulated chickens using this system. Furthermore, deletion of a short region possibly containing the promoter elements (- 2112 to - 1569. bp) completely abrogated oviduct-specific expression. Taken together, these results suggest that weak expression of this putative promoter causes oviduct-specific expression of the transgene.

元の言語英語
ページ(範囲)146-153
ページ数8
ジャーナルJournal of Bioscience and Bioengineering
113
発行部数2
DOI
出版物ステータス出版済み - 2 1 2012

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Oviducts
Hormones
Ovalbumin
Transgenes
Chickens
Deoxyribonuclease I
Growth Hormone
Trans-Activators
Human Growth Hormone
DNA sequences
Packaging
Egg White
Quail
Product Packaging
Deoxyribonucleases

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

これを引用

Chicken oviduct-specific expression of transgene by a hybrid ovalbumin enhancer and the Tet expression system. / Kodama, Daisuke; Nishimiya, Daisuke; Nishijima, Ken ichi; Okino, Yuuki; Inayoshi, Yujin; Kojima, Yasuhiro; Ono, Ken ichiro; Motono, Makoto; Miyake, Katsuhide; Kawabe, Yoshinori; Kyogoku, Kenji; Yamashita, Takashi; Kamihira, Masamichi; Iijima, Shinji.

:: Journal of Bioscience and Bioengineering, 巻 113, 番号 2, 01.02.2012, p. 146-153.

研究成果: ジャーナルへの寄稿記事

Kodama, D, Nishimiya, D, Nishijima, KI, Okino, Y, Inayoshi, Y, Kojima, Y, Ono, KI, Motono, M, Miyake, K, Kawabe, Y, Kyogoku, K, Yamashita, T, Kamihira, M & Iijima, S 2012, 'Chicken oviduct-specific expression of transgene by a hybrid ovalbumin enhancer and the Tet expression system', Journal of Bioscience and Bioengineering, 巻. 113, 番号 2, pp. 146-153. https://doi.org/10.1016/j.jbiosc.2011.10.006
Kodama, Daisuke ; Nishimiya, Daisuke ; Nishijima, Ken ichi ; Okino, Yuuki ; Inayoshi, Yujin ; Kojima, Yasuhiro ; Ono, Ken ichiro ; Motono, Makoto ; Miyake, Katsuhide ; Kawabe, Yoshinori ; Kyogoku, Kenji ; Yamashita, Takashi ; Kamihira, Masamichi ; Iijima, Shinji. / Chicken oviduct-specific expression of transgene by a hybrid ovalbumin enhancer and the Tet expression system. :: Journal of Bioscience and Bioengineering. 2012 ; 巻 113, 番号 2. pp. 146-153.
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abstract = "We generated genetically manipulated chickens and quail by infecting them with a retroviral vector expressing the human growth hormone under the control of chicken ovalbumin promoter/enhancer up to - 3861. bp from the transcriptional start site. The growth hormone was expressed in an oviduct-specific manner and was found in egg white, although its level was low. The DNA sequence of the integrated form of the viral vector in the packaging cells was shown to be truncated and contained only the sequence spanning - 3861 to - 1569. bp. This represented only the DNase I hypersensitive site (DHS) III of the 4 DHSs and lacked the proximal promoter of the ovalbumin control region. We found several TATA-like and other promoter motifs of approximately - 1800. bp and considered that these promoter motifs and DHS III may cause weak but oviduct-specific expression of the growth hormone. To prove this hypothesis and apply this system to oviduct-specific expression of the transgene, the truncated regulatory sequence was fused to an artificial transactivator-promoter system. In this system, initial weak but oviduct-specific expression of the Tet activator from the promoter element in the ovalbumin control sequence triggered a self-amplifying cycle of expression. DsRed was specifically expressed in oviduct cells of genetically manipulated chickens using this system. Furthermore, deletion of a short region possibly containing the promoter elements (- 2112 to - 1569. bp) completely abrogated oviduct-specific expression. Taken together, these results suggest that weak expression of this putative promoter causes oviduct-specific expression of the transgene.",
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AU - Kodama, Daisuke

AU - Nishimiya, Daisuke

AU - Nishijima, Ken ichi

AU - Okino, Yuuki

AU - Inayoshi, Yujin

AU - Kojima, Yasuhiro

AU - Ono, Ken ichiro

AU - Motono, Makoto

AU - Miyake, Katsuhide

AU - Kawabe, Yoshinori

AU - Kyogoku, Kenji

AU - Yamashita, Takashi

AU - Kamihira, Masamichi

AU - Iijima, Shinji

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