Chiral amino acid metabolomics for novel biomarker screening in the prognosis of chronic kidney disease

Tomonori Kimura, Kenji Hamase, Yurika Miyoshi, Ryohei Yamamoto, Keiko Yasuda, Masashi Mita, Hiromi Rakugi, Terumasa Hayashi, Yoshitaka Isaka

研究成果: ジャーナルへの寄稿記事

41 引用 (Scopus)

抄録

D-Amino acids, the enantiomers of L-amino acids, are increasingly recognized as novel biomarkers. Although the amounts of D-amino acids are usually very trace in human, some of them have sporadically been detected in blood from patients with kidney diseases. This study examined whether multiple chiral amino acids would be associated with kidney functions, comorbidities, and prognosis of chronic kidney disease (CKD) by enantioselective analyses of all chiral amino acids with a micro-two-dimensional high-performance liquid chromatograph (2D-HPLC)-based analytical platform. 16 out of 21 D-amino acids were detected in plasma from 108 CKD patients in a longitudinal cohort. The levels of D-Ser, D-Pro, and D-Asn were strongly associated with kidney function (estimated glomerular filtration ratio), the levels of D-Ala and D-Pro were associated with age, and the level of D-Asp and D-Pro were associated with the presence of diabetes mellitus. D-Ser and D-Asn were significantly associated with the progression of CKD in mutually-adjusted Cox regression analyses; the risk of composite end point (developing to ESKD or death before ESKD) was elevated from 2.7- to 3.8-fold in those with higher levels of plasma D-Ser and D-Asn. These findings identified chiral amino acids as potential biomarkers in kidney diseases.

元の言語英語
記事番号26137
ジャーナルScientific reports
6
DOI
出版物ステータス出版済み - 5 18 2016

Fingerprint

Metabolomics
Chronic Renal Insufficiency
Biomarkers
Amino Acids
Kidney Diseases
Kidney
Comorbidity
Diabetes Mellitus
High Pressure Liquid Chromatography
Regression Analysis

All Science Journal Classification (ASJC) codes

  • General

これを引用

Chiral amino acid metabolomics for novel biomarker screening in the prognosis of chronic kidney disease. / Kimura, Tomonori; Hamase, Kenji; Miyoshi, Yurika; Yamamoto, Ryohei; Yasuda, Keiko; Mita, Masashi; Rakugi, Hiromi; Hayashi, Terumasa; Isaka, Yoshitaka.

:: Scientific reports, 巻 6, 26137, 18.05.2016.

研究成果: ジャーナルへの寄稿記事

Kimura, T, Hamase, K, Miyoshi, Y, Yamamoto, R, Yasuda, K, Mita, M, Rakugi, H, Hayashi, T & Isaka, Y 2016, 'Chiral amino acid metabolomics for novel biomarker screening in the prognosis of chronic kidney disease', Scientific reports, 巻. 6, 26137. https://doi.org/10.1038/srep26137
Kimura, Tomonori ; Hamase, Kenji ; Miyoshi, Yurika ; Yamamoto, Ryohei ; Yasuda, Keiko ; Mita, Masashi ; Rakugi, Hiromi ; Hayashi, Terumasa ; Isaka, Yoshitaka. / Chiral amino acid metabolomics for novel biomarker screening in the prognosis of chronic kidney disease. :: Scientific reports. 2016 ; 巻 6.
@article{0f50ad1558ae4ceb8ff834e83e20b808,
title = "Chiral amino acid metabolomics for novel biomarker screening in the prognosis of chronic kidney disease",
abstract = "D-Amino acids, the enantiomers of L-amino acids, are increasingly recognized as novel biomarkers. Although the amounts of D-amino acids are usually very trace in human, some of them have sporadically been detected in blood from patients with kidney diseases. This study examined whether multiple chiral amino acids would be associated with kidney functions, comorbidities, and prognosis of chronic kidney disease (CKD) by enantioselective analyses of all chiral amino acids with a micro-two-dimensional high-performance liquid chromatograph (2D-HPLC)-based analytical platform. 16 out of 21 D-amino acids were detected in plasma from 108 CKD patients in a longitudinal cohort. The levels of D-Ser, D-Pro, and D-Asn were strongly associated with kidney function (estimated glomerular filtration ratio), the levels of D-Ala and D-Pro were associated with age, and the level of D-Asp and D-Pro were associated with the presence of diabetes mellitus. D-Ser and D-Asn were significantly associated with the progression of CKD in mutually-adjusted Cox regression analyses; the risk of composite end point (developing to ESKD or death before ESKD) was elevated from 2.7- to 3.8-fold in those with higher levels of plasma D-Ser and D-Asn. These findings identified chiral amino acids as potential biomarkers in kidney diseases.",
author = "Tomonori Kimura and Kenji Hamase and Yurika Miyoshi and Ryohei Yamamoto and Keiko Yasuda and Masashi Mita and Hiromi Rakugi and Terumasa Hayashi and Yoshitaka Isaka",
year = "2016",
month = "5",
day = "18",
doi = "10.1038/srep26137",
language = "English",
volume = "6",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - Chiral amino acid metabolomics for novel biomarker screening in the prognosis of chronic kidney disease

AU - Kimura, Tomonori

AU - Hamase, Kenji

AU - Miyoshi, Yurika

AU - Yamamoto, Ryohei

AU - Yasuda, Keiko

AU - Mita, Masashi

AU - Rakugi, Hiromi

AU - Hayashi, Terumasa

AU - Isaka, Yoshitaka

PY - 2016/5/18

Y1 - 2016/5/18

N2 - D-Amino acids, the enantiomers of L-amino acids, are increasingly recognized as novel biomarkers. Although the amounts of D-amino acids are usually very trace in human, some of them have sporadically been detected in blood from patients with kidney diseases. This study examined whether multiple chiral amino acids would be associated with kidney functions, comorbidities, and prognosis of chronic kidney disease (CKD) by enantioselective analyses of all chiral amino acids with a micro-two-dimensional high-performance liquid chromatograph (2D-HPLC)-based analytical platform. 16 out of 21 D-amino acids were detected in plasma from 108 CKD patients in a longitudinal cohort. The levels of D-Ser, D-Pro, and D-Asn were strongly associated with kidney function (estimated glomerular filtration ratio), the levels of D-Ala and D-Pro were associated with age, and the level of D-Asp and D-Pro were associated with the presence of diabetes mellitus. D-Ser and D-Asn were significantly associated with the progression of CKD in mutually-adjusted Cox regression analyses; the risk of composite end point (developing to ESKD or death before ESKD) was elevated from 2.7- to 3.8-fold in those with higher levels of plasma D-Ser and D-Asn. These findings identified chiral amino acids as potential biomarkers in kidney diseases.

AB - D-Amino acids, the enantiomers of L-amino acids, are increasingly recognized as novel biomarkers. Although the amounts of D-amino acids are usually very trace in human, some of them have sporadically been detected in blood from patients with kidney diseases. This study examined whether multiple chiral amino acids would be associated with kidney functions, comorbidities, and prognosis of chronic kidney disease (CKD) by enantioselective analyses of all chiral amino acids with a micro-two-dimensional high-performance liquid chromatograph (2D-HPLC)-based analytical platform. 16 out of 21 D-amino acids were detected in plasma from 108 CKD patients in a longitudinal cohort. The levels of D-Ser, D-Pro, and D-Asn were strongly associated with kidney function (estimated glomerular filtration ratio), the levels of D-Ala and D-Pro were associated with age, and the level of D-Asp and D-Pro were associated with the presence of diabetes mellitus. D-Ser and D-Asn were significantly associated with the progression of CKD in mutually-adjusted Cox regression analyses; the risk of composite end point (developing to ESKD or death before ESKD) was elevated from 2.7- to 3.8-fold in those with higher levels of plasma D-Ser and D-Asn. These findings identified chiral amino acids as potential biomarkers in kidney diseases.

UR - http://www.scopus.com/inward/record.url?scp=84970027615&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84970027615&partnerID=8YFLogxK

U2 - 10.1038/srep26137

DO - 10.1038/srep26137

M3 - Article

VL - 6

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 26137

ER -