Chloroplastic glucose 6-phosphate dehydrogenase from Chlorella vulgaris alleviates freezing and menadione-induced oxidative stresses in Saccharomyces cerevisiae

Takeshi Machida, Naoto Ohashi, Ayano Mimura, Ken-ichi Honjoh, Masayoshi Iio, Takahisa Miyamoto

研究成果: Contribution to journalArticle

抜粋

Enhanced glucose 6-phosphate dehydrogenase (E.C.1.1.1.49, G6PDH) activity has been identified as a hardening-induced intracellular change of Chlorella vulgaris, which acquires freezing tolerance during hardening. In the present study, a full-length cDNA clone corresponding to a gene encoding a chloroplastic isoform of G6PDH, designated CvchG6PDH, was isolated from C. vulgaris C-27. By comparing the deduced amino acid sequence of CvchG6PDH with the N-terminal amino acid sequence of mature G6PDH 2 protein isolated previously, a DNA region encoding mature CvchG6PDH was determined and designated mCvchG6PDH. The deduced amino acid sequence of CvchG6PDH showed higher homology to those of plant plastidic G6PDH genes than those of cytosolic ones. A recombinant mCvchG6PDH protein expressed in Escherichia coli showed similar enzymatic properties to previously-isolated Chlorella G6PDH 2, suggesting that the gene encoded plastidic G6PDH 2 protein. Expression of CvchG6PDH was induced transcriptionally throughout 24-h hardening, while the translation was induced up to 9-h hardening and then decreased, and the change did not reflect the enhanced G6PDH activity during hardening. Furthermore, the mCvch-G6PDH alleviated both freezing and menadione-induced oxidative stresses in yeast. We showed the contribution of CvchG6PDH in menadione stress tolerance as one of its functions in the acquisition of freezing tolerance of Chlorella.

元の言語英語
ページ(範囲)29-38
ページ数10
ジャーナルJournal of the Faculty of Agriculture, Kyushu University
55
発行部数1
出版物ステータス出版済み - 2 2010
外部発表Yes

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Biotechnology

フィンガープリント Chloroplastic glucose 6-phosphate dehydrogenase from <i>Chlorella vulgaris</i> alleviates freezing and menadione-induced oxidative stresses in <i>Saccharomyces cerevisiae</i>' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

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