Cholesterol metabolism in ExHC (exogenous hypercholesterolemic) rats

Katsumi Imaizumi, Akihiro Nagatomi, Masao Sato, Akira Tominaga, Michihiro Sugano

研究成果: ジャーナルへの寄稿記事

19 引用 (Scopus)

抄録

Exogenous hypercholesterolemic (ExHC) rats, that develop hypercholesterolemia for exogenous cholesterol, are an established strain Isolated from Sprague-Dawley (SD) rats by Imai and Matsumura ((1973) Atherosclerosis, 18, 59-64). The present study was carried out to clarify the cause of hyperresponsivity in ExHC rats to dietary cholesterol. As early as one day after feeding a high cholesterol diet (1%) serum cholesterol level was doubled in ExHC rats, while the level of hepatic cholesterol was two-thirds of SD rats. The elevation of serum cholesterol was mainly attributed to the d < 1.006 g/ml fractions. Cholesterol feeding increased fecal bile acid excretion in both strains, but to a more greater extent in SD rats. Absorption of dietary cholesterol and synthesis of cholesterol in vivo were similar between the strains. The uptake of β-very-low-density-lipoproteins (β-VLDL) in vivo and the primary cultured hepatocytes was lower in ExHC rats, when a high-cholesterol diet was fed. Even without feeding of a high-cholesterol diet, preincubation with cholesterol-rich lipoproteins caused a lower association and degradation of β-VLDL by the hepatocytes from ExHC rats. Incubation of hepatocytes with cholesterol-rich lipoproteins did not affect the secretion of [14C]cholesterol into the density less than 1.006 g/ml fraction, but suppressed the secretion into the medium density greater than 1.006 g/ml fractions. These results suggest that ExHC rats, as compared to SD rats, are defective of hepatic uptake and processing cholesterol to bile acids.

元の言語英語
ページ(範囲)101-109
ページ数9
ジャーナルBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
1123
発行部数1
DOI
出版物ステータス出版済み - 1 3 1992

Fingerprint

Metabolism
Rats
Cholesterol
Sprague Dawley Rats
Dietary Cholesterol
Nutrition
Hepatocytes
Diet
Bile Acids and Salts
Lipoproteins
VLDL Lipoproteins
Liver
Hypercholesterolemia
Serum
Atherosclerosis
Association reactions
Degradation

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Endocrinology

これを引用

Cholesterol metabolism in ExHC (exogenous hypercholesterolemic) rats. / Imaizumi, Katsumi; Nagatomi, Akihiro; Sato, Masao; Tominaga, Akira; Sugano, Michihiro.

:: Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism, 巻 1123, 番号 1, 03.01.1992, p. 101-109.

研究成果: ジャーナルへの寄稿記事

Imaizumi, Katsumi ; Nagatomi, Akihiro ; Sato, Masao ; Tominaga, Akira ; Sugano, Michihiro. / Cholesterol metabolism in ExHC (exogenous hypercholesterolemic) rats. :: Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism. 1992 ; 巻 1123, 番号 1. pp. 101-109.
@article{40c9d13891b746409b2c8ad220bc54b7,
title = "Cholesterol metabolism in ExHC (exogenous hypercholesterolemic) rats",
abstract = "Exogenous hypercholesterolemic (ExHC) rats, that develop hypercholesterolemia for exogenous cholesterol, are an established strain Isolated from Sprague-Dawley (SD) rats by Imai and Matsumura ((1973) Atherosclerosis, 18, 59-64). The present study was carried out to clarify the cause of hyperresponsivity in ExHC rats to dietary cholesterol. As early as one day after feeding a high cholesterol diet (1{\%}) serum cholesterol level was doubled in ExHC rats, while the level of hepatic cholesterol was two-thirds of SD rats. The elevation of serum cholesterol was mainly attributed to the d < 1.006 g/ml fractions. Cholesterol feeding increased fecal bile acid excretion in both strains, but to a more greater extent in SD rats. Absorption of dietary cholesterol and synthesis of cholesterol in vivo were similar between the strains. The uptake of β-very-low-density-lipoproteins (β-VLDL) in vivo and the primary cultured hepatocytes was lower in ExHC rats, when a high-cholesterol diet was fed. Even without feeding of a high-cholesterol diet, preincubation with cholesterol-rich lipoproteins caused a lower association and degradation of β-VLDL by the hepatocytes from ExHC rats. Incubation of hepatocytes with cholesterol-rich lipoproteins did not affect the secretion of [14C]cholesterol into the density less than 1.006 g/ml fraction, but suppressed the secretion into the medium density greater than 1.006 g/ml fractions. These results suggest that ExHC rats, as compared to SD rats, are defective of hepatic uptake and processing cholesterol to bile acids.",
author = "Katsumi Imaizumi and Akihiro Nagatomi and Masao Sato and Akira Tominaga and Michihiro Sugano",
year = "1992",
month = "1",
day = "3",
doi = "10.1016/0005-2760(92)90176-V",
language = "English",
volume = "1123",
pages = "101--109",
journal = "Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids",
issn = "1388-1981",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Cholesterol metabolism in ExHC (exogenous hypercholesterolemic) rats

AU - Imaizumi, Katsumi

AU - Nagatomi, Akihiro

AU - Sato, Masao

AU - Tominaga, Akira

AU - Sugano, Michihiro

PY - 1992/1/3

Y1 - 1992/1/3

N2 - Exogenous hypercholesterolemic (ExHC) rats, that develop hypercholesterolemia for exogenous cholesterol, are an established strain Isolated from Sprague-Dawley (SD) rats by Imai and Matsumura ((1973) Atherosclerosis, 18, 59-64). The present study was carried out to clarify the cause of hyperresponsivity in ExHC rats to dietary cholesterol. As early as one day after feeding a high cholesterol diet (1%) serum cholesterol level was doubled in ExHC rats, while the level of hepatic cholesterol was two-thirds of SD rats. The elevation of serum cholesterol was mainly attributed to the d < 1.006 g/ml fractions. Cholesterol feeding increased fecal bile acid excretion in both strains, but to a more greater extent in SD rats. Absorption of dietary cholesterol and synthesis of cholesterol in vivo were similar between the strains. The uptake of β-very-low-density-lipoproteins (β-VLDL) in vivo and the primary cultured hepatocytes was lower in ExHC rats, when a high-cholesterol diet was fed. Even without feeding of a high-cholesterol diet, preincubation with cholesterol-rich lipoproteins caused a lower association and degradation of β-VLDL by the hepatocytes from ExHC rats. Incubation of hepatocytes with cholesterol-rich lipoproteins did not affect the secretion of [14C]cholesterol into the density less than 1.006 g/ml fraction, but suppressed the secretion into the medium density greater than 1.006 g/ml fractions. These results suggest that ExHC rats, as compared to SD rats, are defective of hepatic uptake and processing cholesterol to bile acids.

AB - Exogenous hypercholesterolemic (ExHC) rats, that develop hypercholesterolemia for exogenous cholesterol, are an established strain Isolated from Sprague-Dawley (SD) rats by Imai and Matsumura ((1973) Atherosclerosis, 18, 59-64). The present study was carried out to clarify the cause of hyperresponsivity in ExHC rats to dietary cholesterol. As early as one day after feeding a high cholesterol diet (1%) serum cholesterol level was doubled in ExHC rats, while the level of hepatic cholesterol was two-thirds of SD rats. The elevation of serum cholesterol was mainly attributed to the d < 1.006 g/ml fractions. Cholesterol feeding increased fecal bile acid excretion in both strains, but to a more greater extent in SD rats. Absorption of dietary cholesterol and synthesis of cholesterol in vivo were similar between the strains. The uptake of β-very-low-density-lipoproteins (β-VLDL) in vivo and the primary cultured hepatocytes was lower in ExHC rats, when a high-cholesterol diet was fed. Even without feeding of a high-cholesterol diet, preincubation with cholesterol-rich lipoproteins caused a lower association and degradation of β-VLDL by the hepatocytes from ExHC rats. Incubation of hepatocytes with cholesterol-rich lipoproteins did not affect the secretion of [14C]cholesterol into the density less than 1.006 g/ml fraction, but suppressed the secretion into the medium density greater than 1.006 g/ml fractions. These results suggest that ExHC rats, as compared to SD rats, are defective of hepatic uptake and processing cholesterol to bile acids.

UR - http://www.scopus.com/inward/record.url?scp=0026573017&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026573017&partnerID=8YFLogxK

U2 - 10.1016/0005-2760(92)90176-V

DO - 10.1016/0005-2760(92)90176-V

M3 - Article

C2 - 1730041

AN - SCOPUS:0026573017

VL - 1123

SP - 101

EP - 109

JO - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids

JF - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids

SN - 1388-1981

IS - 1

ER -