Circular permutation of ligand-binding module improves dynamic range of genetically encoded FRET-based nanosensor

Satoshi Okada, Kazuhisa Ota, Takashi Ito

研究成果: ジャーナルへの寄稿記事

25 引用 (Scopus)

抄録

Quantitative measurement of small molecules with high spatiotemporal resolution provides a solid basis for correct understanding and accurate modeling of metabolic regulation. A promising approach toward this goal is the FLIP (fluorescent indicator protein) nanosensor based on bacterial periplasmic binding proteins (PBPs) and fluorescence resonance energy transfer (FRET) between the yellow and cyan variants of green fluorescent protein (GFP). Each FLIP has a PBP module that specifically binds its ligand to induce a conformation change, leading to a change in FRET between the two GFP variant modules attached to the N- and C-termini of the PBP. The larger is the dynamic range the more reliable is the measurement. Thus, we attempted to expand the dynamic range of FLIP by introducing a circular permutation with a hinge loop deletion to the PBP module. All the six circularly permutated PBPs tested, including structurally distinct Type I and Type II PBPs, showed larger dynamic ranges than their respective native forms when used for FLIP. Notably, the circular permutation made three PBPs, which totally failed to show FRET change when used as their native forms, fully capable of functioning as a ligand binding module of FLIP. These FLIPs were successfully used for the determination of amino acid concentration in complex solutions as well as real-time measurement of amino acid influx in living yeast cells. Thus, the circular permutation strategy would not only improve the performance of each nanosensor but also expand the repertoire of metabolites that can be measured by the FLIP nanosensor technology. Published by Wiley-Blackwell.

元の言語英語
ページ(範囲)2518-2527
ページ数10
ジャーナルProtein Science
18
発行部数12
DOI
出版物ステータス出版済み - 12 1 2009

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Periplasmic Binding Proteins
Nanosensors
Fluorescence Resonance Energy Transfer
Ligands
Proteins
Green Fluorescent Proteins
Amino Acids
Bacterial Proteins
Hinges
Metabolites
Time measurement
Yeast
Conformations
Yeasts
Cells
Technology
Molecules

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

これを引用

Circular permutation of ligand-binding module improves dynamic range of genetically encoded FRET-based nanosensor. / Okada, Satoshi; Ota, Kazuhisa; Ito, Takashi.

:: Protein Science, 巻 18, 番号 12, 01.12.2009, p. 2518-2527.

研究成果: ジャーナルへの寄稿記事

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