Circulating intestine-derived exosomal miR-328 in plasma, a possible biomarker for estimating BCRP function in the human intestines

Keisuke Gotanda, Takeshi Hirota, Jumpei Saito, Masato Fukae, Yu Egashira, Noritomo Izumi, Mariko Deguchi, Miyuki Kimura, Shunji Matsuki, Shin Irie, Ichiro Ieiri

研究成果: ジャーナルへの寄稿記事

8 引用 (Scopus)

抄録

A variant in the breast cancer resistance protein (BCRP) gene, 421C> A is a useful biomarker for describing large inter-individual differences in the pharmacokinetics of sulfasalazine (SASP), a BCRP substrate. However, large intra-genotypic variability still exists in spite of the incorporation of this variant into the pharmacokinetics of SASP. Since miR-328 negatively regulates BCRP expression in human tissues, we hypothesized that exosomal miR-328 in plasma, which leaks from the intestines, is a possible biomarker for estimating BCRP activity in the human intestines. We established an immunoprecipitation-based quantitative method for circulating intestine-derived miR-328 in plasma using an anti-glycoprotein A33 antibody. A clinical study was conducted with an open-label, non-randomized, and single-arm design involving 33 healthy participants. Intestine-derived exosomal miR-328 levels positively correlated (P < 0.05) with SASP AUC 0-48, suggesting that subjects with high miR-328 levels have low intestinal BCRP activity, resulting in the high AUC of SASP. Circulating intestine-derived exosomal miR-328 in plasma has potential as a possible biomarker for estimating BCRP function in the human intestines.

元の言語英語
記事番号32299
ジャーナルScientific reports
6
DOI
出版物ステータス出版済み - 8 30 2016

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Intestines
Biomarkers
Breast Neoplasms
Proteins
Area Under Curve
Pharmacokinetics
Intestinal Neoplasms
losigame
Sulfasalazine
Immunoprecipitation
Human Activities
Individuality
Glycoproteins
Healthy Volunteers
Antibodies

All Science Journal Classification (ASJC) codes

  • General

これを引用

Circulating intestine-derived exosomal miR-328 in plasma, a possible biomarker for estimating BCRP function in the human intestines. / Gotanda, Keisuke; Hirota, Takeshi; Saito, Jumpei; Fukae, Masato; Egashira, Yu; Izumi, Noritomo; Deguchi, Mariko; Kimura, Miyuki; Matsuki, Shunji; Irie, Shin; Ieiri, Ichiro.

:: Scientific reports, 巻 6, 32299, 30.08.2016.

研究成果: ジャーナルへの寄稿記事

Gotanda, Keisuke ; Hirota, Takeshi ; Saito, Jumpei ; Fukae, Masato ; Egashira, Yu ; Izumi, Noritomo ; Deguchi, Mariko ; Kimura, Miyuki ; Matsuki, Shunji ; Irie, Shin ; Ieiri, Ichiro. / Circulating intestine-derived exosomal miR-328 in plasma, a possible biomarker for estimating BCRP function in the human intestines. :: Scientific reports. 2016 ; 巻 6.
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abstract = "A variant in the breast cancer resistance protein (BCRP) gene, 421C> A is a useful biomarker for describing large inter-individual differences in the pharmacokinetics of sulfasalazine (SASP), a BCRP substrate. However, large intra-genotypic variability still exists in spite of the incorporation of this variant into the pharmacokinetics of SASP. Since miR-328 negatively regulates BCRP expression in human tissues, we hypothesized that exosomal miR-328 in plasma, which leaks from the intestines, is a possible biomarker for estimating BCRP activity in the human intestines. We established an immunoprecipitation-based quantitative method for circulating intestine-derived miR-328 in plasma using an anti-glycoprotein A33 antibody. A clinical study was conducted with an open-label, non-randomized, and single-arm design involving 33 healthy participants. Intestine-derived exosomal miR-328 levels positively correlated (P < 0.05) with SASP AUC 0-48, suggesting that subjects with high miR-328 levels have low intestinal BCRP activity, resulting in the high AUC of SASP. Circulating intestine-derived exosomal miR-328 in plasma has potential as a possible biomarker for estimating BCRP function in the human intestines.",
author = "Keisuke Gotanda and Takeshi Hirota and Jumpei Saito and Masato Fukae and Yu Egashira and Noritomo Izumi and Mariko Deguchi and Miyuki Kimura and Shunji Matsuki and Shin Irie and Ichiro Ieiri",
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AU - Egashira, Yu

AU - Izumi, Noritomo

AU - Deguchi, Mariko

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