Clinical trial of the intratumoral administration of labeled DC combined with systemic chemotherapy for esophageal cancer

Shinichi Fujiwara, Hisashi Wada, Hiroshi Miyata, Junji Kawada, Ryohei Kawabata, Hiroyoshi Nishikawa, Sacha Gnjatic, Christine Sedrak, Eiichi Sato, Yurika Nakamura, Mitsuru Sakakibara, Tatsuya Kanto, Eku Shimosegawa, Jun Hatazawa, Tsuyoshi Takahashi, Yukinori Kurokawa, Makoto Yamasaki, Kiyokazu Nakajima, Shuji Takiguchi, Eiichi NakayamaMasaki Mori, Yuichiro Doki

研究成果: Contribution to journalArticle査読

34 被引用数 (Scopus)

抄録

Esophageal cancer is a highly aggressive disease, and improved modalities for its treatment are needed. We performed chemoimmunotherapy involving the intratumoral administration of In-labeled dendritic cells (DC) in combination with preoperative chemotherapy in 5 esophageal cancer patients. Mature DC were generated and traced by scintigraphy after their administration. No adverse events that were directly related to the intratumoral DC administration were observed. Delayed-type hypersensitivity skin tests against keyhole limpet hemocyanin, which was added to the culture medium, detected a positive response in 3 patients, and keyhole limpet hemocyanin antibody production was observed in 4 patients, suggesting that intratumorally administered DC migrate to the lymph nodes, where they function as antigen-presenting cells. However, scintigraphic images obtained after the DC administration demonstrated that the DC remained at the esophageal tumor injection sites in all cases, and no DC accumulation was observed elsewhere. The accumulation of CD83 cells in the primary tumor was also observed in 2 out of 4 patients in an immunohistochemical analysis using surgically resected specimens. Although the induction of tumor-specific immune responses during chemoimmunotherapy was also analyzed in enzyme-linked immunosorbent assay against 28 tumor antigens, none of the antibodies against the antigens displayed enhanced titers. No changes of NY-ESO-1-specific cellular immune response was observed in a patient who displayed NY-ESO-1 antibody production before the DC administration. These results suggest that the intratumoral administration of In-labeled mature DC during chemotherapy does not lead to detectable DC migration from the primary tumor to the draining lymph nodes, and therefore, might not achieve an optimal clinical response.

本文言語英語
ページ(範囲)513-521
ページ数9
ジャーナルJournal of Immunotherapy
35
6
DOI
出版ステータス出版済み - 7 2012
外部発表はい

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Pharmacology
  • Cancer Research

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