Clofibrate-inducible, 28-kDa peroxisomal integral membrane protein is encoded by PEX11

Isao Abe, Kanji Okumoto, Shigehiko Tamura, Yukio Fujiki

研究成果: ジャーナルへの寄稿記事

58 引用 (Scopus)

抄録

We cloned a human PEX11 cDNA by expressed sequence tag homology search using yeast Candida boidinii PEX11, followed by screening of human liver cDNA library. PEX11 encoded a peroxisomal protein Pex11p comprising 247 amino acids, with two transmembrane segments and a dilysine motif at the C-termius. Pex11p comigrated in SDS-PAGE with a 28-kDA peroxisomal integral membrane protein (PMP28) isolated from the liver of clofibrate-treated rats and was crossreactive to anti-PMP28 antibody, thereby indicating PEX11 to encode PMP28. Pex11p exposes both N- and C-terminal parts to the cytosol. PEX11 was not responsible for ten complementation groups of human peroxisome deficiency disorders.

元の言語英語
ページ(範囲)468-472
ページ数5
ジャーナルFEBS Letters
431
発行部数3
DOI
出版物ステータス出版済み - 7 24 1998

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Clofibrate
Liver
lysyllysine
Membrane Proteins
Candida
Expressed Sequence Tags
Gene Library
Yeast
Rats
Anti-Idiotypic Antibodies
Screening
Peroxisomes
Complementary DNA
Sequence Homology
Amino Acids
Cytosol
Polyacrylamide Gel Electrophoresis
Yeasts
Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Molecular Biology

これを引用

Clofibrate-inducible, 28-kDa peroxisomal integral membrane protein is encoded by PEX11. / Abe, Isao; Okumoto, Kanji; Tamura, Shigehiko; Fujiki, Yukio.

:: FEBS Letters, 巻 431, 番号 3, 24.07.1998, p. 468-472.

研究成果: ジャーナルへの寄稿記事

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