Clonal origin of Epstein-Barr virus (EBV)-infected T/NK-cell subpopulations in EBV-positive T/NK-cell lymphoproliferative disorders of childhood

Shouichi Ohga, Masataka Ishimura, Goichi Yoshimoto, Toshihiro Miyamoto, Hidetoshi Takada, Tamami Tanaka, Koichi Ohshima, Yoshiyasu Ogawa, Ken Ichi Imadome, Yasunobu Abe, Koichi Akashi, Toshiro Hara

研究成果: Contribution to journalArticle査読

15 被引用数 (Scopus)

抄録

Background: In Japan, chronic active Epstein-Barr virus infection (CAEBV) may manifest with infection of T-cells or NK-cells, clonal lymphoid proliferations, and overt lymphoid malignancy. These EBV-positive lymphoproliferative disorders (EBV +LPD) of childhood are related to, but distinct from the infectious mononucleosis-like CAEBV seen in Western populations. The clonal nature of viral infection within lymphoid subsets of patients with EBV +LPD of childhood is not well described. Objectives: Viral distribution and clonotype were assessed within T-cell subsets, NK-cells, and CD34 +stem cells following high purity cell sorting. Study design: Six Japanese patients with EBV +LPD of childhood (3 T-cell LPD and 3 NK-cell LPD) were recruited. Prior to immunochemotherapy, viral loads and clonal analyses of T-cell subsets, NK-cells, and CD34 +stem cells were studied by high-accuracy cell sorting (>99.5%), Southern blotting and real-time polymerase chain reaction. Results: Patient 1 had a monoclonal proliferation of EBV-infected γδT-cells and carried a lower copy number of EBV in αβT-cells. Patients 2 and 3 had clonal expansions of EBV-infected CD4 +T-cells, and lower EBV load in NK-cells. Patients 4, 5 and 6 had EBV +NK-cell expansions with higher EBV load than T-cells. EBV-terminal repeats were determined as clonal bands in the minor targeted populations of 5 patients. The size of terminal repeats indicated the same clonotype in minor subsets as in the major subsets of four patients. EBV was not, however, detected in the bone marrow-derived CD34 +stem cells of patients. Conclusions: A single EBV clonotype may infect multiple NK-cell and T-cell subsets of patients with EBV +LPD of childhood. CD34 +stem cells are spared, suggesting infection of more differentiated elements.

本文言語英語
ページ(範囲)31-37
ページ数7
ジャーナルJournal of Clinical Virology
51
1
DOI
出版ステータス出版済み - 5 2011

All Science Journal Classification (ASJC) codes

  • Virology
  • Infectious Diseases

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