TY - JOUR
T1 - Cloning and characterization of a gene encoding a new thermostable hemolysin from Vibrio parahaemolyticus
AU - Taniguchi, Hatsumi
AU - Kubomura, Shigeo
AU - Hirano, Hideaki
AU - Mizue, Kumiko
AU - Ogawa, Midori
AU - Mizuguchi, Yasuo
N1 - Funding Information:
This study was supported by a grant p~rr ovided by the Ministry of Education, Science, aann d Culture of the Japanese Goverment (No. 662233(0 4036), and by a grant from Yakult Co. LTD (('T okyo, Japan). We thank K. Ogata, and S. OOhhttoolm o for supplying the F'ibrio strains.
PY - 1990/2
Y1 - 1990/2
N2 - A new thermostable hemolsin (σ-VPH) gene was cloned from a Kanagawa-negative Vibrio parahaemolyticus strain into vector pBR322 in Escherichia coli K12. The nucleotide and amino acid sequences had np homology with those of the thermostable direct hemolysin (TDH) which causes the Kanagawa phenomenon, and of the thermolabile hemolysin (TLH) of V. parahaemolyticus. The gene was present in all V. parahaemolyticus strains tested and also in one strain of V. damsela.
AB - A new thermostable hemolsin (σ-VPH) gene was cloned from a Kanagawa-negative Vibrio parahaemolyticus strain into vector pBR322 in Escherichia coli K12. The nucleotide and amino acid sequences had np homology with those of the thermostable direct hemolysin (TDH) which causes the Kanagawa phenomenon, and of the thermolabile hemolysin (TLH) of V. parahaemolyticus. The gene was present in all V. parahaemolyticus strains tested and also in one strain of V. damsela.
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U2 - 10.1016/0378-1097(90)90020-Q
DO - 10.1016/0378-1097(90)90020-Q
M3 - Article
C2 - 2323552
AN - SCOPUS:0025378558
VL - 67
SP - 339
EP - 345
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
SN - 0378-1097
IS - 3
ER -