Cloning and characterization of testis-specific tektin in Bombyx mori

Atsuko Ota, Takahiro Kusakabe, Yasushi Sugimoto, Masateru Takahashi, Yumiko Nakajima, Yutaka Kawaguchi, Katsumi Koga

研究成果: ジャーナルへの寄稿記事

22 引用 (Scopus)

抄録

A testis-specific cDNA library of Bombyx mori was constructed by an mRNA subtraction technique. Several clones were randomly selected and determined for their nucleotide sequences. One of them, designated as BmTST, contained a 3′-part of an open reading frame homologous to tektin, the protein known to form filamentous polymers in the walls of ciliary and flagellar microtubules. Also isolated was a genomic fragment, which contains the 5′-part of the coding sequence of BmTST and its promoter region. As a whole, the complete open reading frame was found to encode 508 amino acid residues, whose sequence had 28, 28 and 30% identities with the Strongylocentrotus purpuratus tektins A1, B1 and C1, respectively. Expression analysis by reverse transcription polymerase chain reaction with the cDNA and Western blotting with a polyclonal antibody indicated that the BmTST gene was expressed specifically in the testis during sperm maturation. The protein was immunologically detected exclusively in the fraction expected to contain the 9+2 flagellar axonemes of sperms. We infer that the BmTst protein is possibly involved in the spermatogenesis of B. mori.

元の言語英語
ページ(範囲)371-382
ページ数12
ジャーナルComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
133
発行部数3
DOI
出版物ステータス出版済み - 11 1 2002

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Bombyx
Cloning
Testis
Organism Cloning
Open Reading Frames
Proteins
Polymerase chain reaction
Subtraction Technique
Strongylocentrotus purpuratus
Sperm Maturation
Transcription
Axoneme
Gene Library
Genetic Promoter Regions
Polymers
Spermatogenesis
Nucleotides
Complementary DNA
Genes
Microtubules

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology
  • Molecular Biology

これを引用

Cloning and characterization of testis-specific tektin in Bombyx mori. / Ota, Atsuko; Kusakabe, Takahiro; Sugimoto, Yasushi; Takahashi, Masateru; Nakajima, Yumiko; Kawaguchi, Yutaka; Koga, Katsumi.

:: Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, 巻 133, 番号 3, 01.11.2002, p. 371-382.

研究成果: ジャーナルへの寄稿記事

Ota, Atsuko ; Kusakabe, Takahiro ; Sugimoto, Yasushi ; Takahashi, Masateru ; Nakajima, Yumiko ; Kawaguchi, Yutaka ; Koga, Katsumi. / Cloning and characterization of testis-specific tektin in Bombyx mori. :: Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology. 2002 ; 巻 133, 番号 3. pp. 371-382.
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