Cloning and expression of gene encoding a novel endoglycoceramidase of Rhodococcus sp. strain C9

Keishi Sakaguchi, Nozomu Okino, Noriyuki Sueyoshi, Hiroyuki Izu, Makoto Ito

研究成果: Contribution to journalArticle査読

9 被引用数 (Scopus)

抄録

Endoglycoceramidase (EGCase) is an enzyme capable of cleaving the glycosidic linkage between oligosaccharides and ceramides of various glycosphingolipids. We previously reported that the Asn-Glu-Pro (NEP) sequence is part of the active site of EGCase of Rhodococcus sp. strain M- 777. This paper describes the molecular cloning of a new EGCase gene utilizing the NEP sequence from the genomic library of Rhodococcus sp. strain C9, which was clearly distinguishable from M-777 by 16S rDNA analysis. C9 EGCase possessed an open reading frame of 1,446 bp encoding 482 amino acids, and showed 78% and 76% identity to M-777 EGCase II at the nucleotide and amino acid levels, respectively. Interestingly, C9 EGCase showed the different specificity to the M-777 enzyme: it hydrolyzed b-series gangliotetraosylceramides more slowly than the M-777 enzyme, whereas both enzymes hydrolyzed a-series gangliosides and neutral glycosphingolipids to the same extent.

本文言語英語
ページ(範囲)145-152
ページ数8
ジャーナルJournal of biochemistry
128
1
DOI
出版ステータス出版済み - 1 1 2000

All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学

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