Cloning and expression of human endothelin-1 receptor cDNA

Kiminori Hosoda, Kazuwa Nakao, Hiroshi-Arai, Shin ichi Suga, Yoshihiro Ogawa, Masashi Mukoyama, Gotaro Shirakami, Yoshihiko Saito, Shigetada Nakanishi, Hiroo Imura

研究成果: Contribution to journalArticle査読

268 被引用数 (Scopus)

抄録

We isolated a human endothelin-1 (ET-1) receptor cDNA from a human placenta cDNA library. The cDNA encodes a 427-amino acid protein with seven putative transmembrane domains. The rank order of the binding to the receptor expressed in COS-7 cells was: ET-1 ≥ ET-2 ≫ ET-3. The receptor expressed in Xenopus oocytes showed a potent electrophysiological response to 1 × 10-7 M ET-1 under voltage clamp at -60 mV, while a much weaker response was produced by 1 × 10-7 M ET-3. Northern blot analysis with RNA from human tissues revealed a single band with a size of 4.3 kb in a wide variety of human tissues, especially highly in the blood vessel.

本文言語英語
ページ(範囲)23-26
ページ数4
ジャーナルFEBS Letters
287
1-2
DOI
出版ステータス出版済み - 8 5 1991
外部発表はい

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

フィンガープリント 「Cloning and expression of human endothelin-1 receptor cDNA」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル